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分子别名（Synonym）
TIGIT, VSIG9, VSTM3
表达区间及表达系统（Source）
PE-Labeled Human TIGIT Protein, His Tag (TIT-HP2H3) is expressed from human 293 cells (HEK293). It contains AA Met 22 - Pro 141 (Accession # Q495A1-1).
Predicted N-terminus: Met 22
蛋白结构（Molecular Characterization）
This protein carries a polyhistidine tag at the C-terminus.
偶联（Conjugate）
PE
Excitation Wavelength: 488 nm / 561 nm
Emission Wavelength: 575 nm
制剂（Formulation）
Lyophilized from 0.22 μm filtered solution in PBS, 0.2% BSA, pH7.4 with trehalose as protectant.
Contact us for customized product form or formulation.
重构方法（Reconstitution）
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
存储（Storage）
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please protect from light and avoid repeated freeze-thaw cycles.
This product is stable after storage at:
- -20°C to -70°C for 12 months in lyophilized state;
- -70°C for 3 months under sterile conditions after reconstitution.
质量管理控制体系（QMS）
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数据展示
CAR阳性表达率检测（Evaluation of CAR expression）
FACS Analysis of TIGIT binding to 293T overexpressing CD155 cells
Flow cytometric analysis of 293T overexpressing CD155 cells staining with PE-Labeled Human TIGIT Protein, His Tag (Cat. No. TIT-HP2H3) at 1:50 dilution (2 μL of the stock solution corresponds to labeling of 5e5 cells in a final volume of 100 µL), compared with negative control protein(Fig. B and A). PE signal was used to evaluate the binding activity (QC tested).
Protocol
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