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Canine TIGIT Protein, His Tag (MALS verified)

COADS MS
DS
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货号-规格
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TIT-C52H7-100ug
¥3675.00
TIT-C52H7-1mg
¥24255.00
合计0件 产品金额¥ 0
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产品详情

  • 分子别名（Synonym）

    TIGIT, VSIG9, VSTM3

  • 表达区间及表达系统（Source）

    Canine TIGIT, His Tag (TIT-C52H7) is expressed from human 293 cells (HEK293). It contains AA Phe 17 - Pro 208 (Accession # F1PKJ0-1).

    Predicted N-terminus: Phe 17

    Request for sequence

  • 蛋白结构（Molecular Characterization）

    TIGIT Structure

    Other Tags and Version Biotin & Other Labeled Version

    This protein carries a polyhistidine tag at the C-terminus.

    The protein has a calculated MW of 23.1 kDa. The protein migrates as 25-33 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

  • 内毒素（Endotoxin）

    Less than 1.0 EU per μg by the LAL method / rFC method.

  • 纯度（Purity）

    >90% as determined by SDS-PAGE.

    >95% as determined by SEC-MALS.

  • 制剂（Formulation）

    Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.

    Contact us for customized product form or formulation.

  • 重构方法（Reconstitution）

    Please see Certificate of Analysis for specific instructions.

    For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

  • 存储（Storage）

    For long term storage, the product should be stored at lyophilized state at -20°C or lower.

    Please avoid repeated freeze-thaw cycles.

    This product is stable after storage at:

    1. -20°C to -70°C for 12 months in lyophilized state;
    2. -70°C for 3 months under sterile conditions after reconstitution.

  • 质量管理控制体系（QMS）

    1. 质量管理体系（ISO, GMP）
    2. 质量优势
    3. 质控流程

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数据展示

  • 电泳（SDS-PAGE）

    TIGIT SDS-PAGE

    Canine TIGIT, His Tag on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 90%.

  • SEC-MALS

    TIGIT SEC-MALS

    The purity of Canine TIGIT, His Tag (Cat. No. TIT-C52H7) is more than 95% and the molecular weight of this protein is around 23-35 kDa verified by SEC-MALS.

    Report

  • 活性（Bioactivity）-ELISA

    TIGIT ELISA

    Immobilized Canine TIGIT, His Tag (Cat. No. TIT-C52H7) at 5 μg/mL (100 μL/well) can bind Human CD155, mouse IgG2a Fc tag, low endotoxin (Cat. No. CD5-H5254) with a linear range of 0.01-0.313 μg/mL (QC tested).

    Protocol

  • 活性（Bioactivity）-BLI

    TIGIT BLI

    Loaded Human CD155, Fc Tag (Cat. No. CD5-H5251) on Protein A Biosensor, can bind Canine TIGIT, His Tag (Cat. No. TIT-C52H7) with an affinity constant of 5 μM as determined in BLI assay (ForteBio Octet Red96e) (Routinely tested).

    Protocol

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背景介绍

T-cell immunoreceptor with Ig and ITIM domains (TIGIT) is also known as V-set and immunoglobulin domain-containing protein 9 (VSIG9), V-set and transmembrane domain-containing protein 3 (VSTM3),which belongs to single-pass type I membrane protein containing an immunoglobulin variable domain, a transmembrane domain and an immunoreceptor tyrosine-based inhibitory motif (ITIM). TIGIT is expressed at low levels on peripheral memory and regulatory CD4+ T-cells and NK cells and is up-regulated following activation of these cells (at protein level). TIGIT binds with high affinity to the poliovirus receptor (PVR) which causes increased secretion of IL10 and decreased secretion of IL12B and suppresses T-cell activation by promoting the generation of mature immunoregulatory dendritic cells.

前沿进展

 
药物研发进展
  • 英文全称：

    T cell immunoglobulin and ITIM domains

  • 中文全称：

    T细胞免疫球蛋白ITIM结构域

  • 种类：

  • 上市药物数量：

    0 详情

  • 临床药物数量：

    38 详情

  • 最高研发阶段：

    临床三期

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