产品详情
分子别名(Synonym)
CEDLAP, TGF-beta 1, TGFB1, DPD1, TGF-beta-1, TGFB
表达区间及表达系统(Source)
Biotinylated Human TGF-Beta 1, Avitag (TG1-H8217) is expressed from human 293 cells (HEK293). It contains AA Ala 279 - Ser 390 (Accession # P01137-1).
Predicted N-terminus: Ala 279
Request for sequence蛋白结构(Molecular Characterization)

Other Tags and Version Biotin & Other Labeled Version
This protein carries an Avi tag (Avitag™) at the C-terminus.
The protein has a calculated MW of 14.5 kDa. The protein migrates as 14-15 kDa under reducing (R) condition, and 24-25 kDa under non-reducing (NR) condition (SDS-PAGE) due to glycosylation.
标记(Labeling)
Biotinylation of this product is performed using Avitag™ technology. Briefly, the single lysine residue in the Avitag is enzymatically labeled with biotin.
蛋白标记度(Protein Ratio)
Passed as determined by the HABA assay / binding ELISA.
纯度(Purity)
>95% as determined by SDS-PAGE.
制剂(Formulation)
Lyophilized from 0.22 μm filtered solution in 50 mM HAC, pH2.9 with trehalose as protectant.
Contact us for customized product form or formulation.
重构方法(Reconstitution)
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
存储(Storage)
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
- -20°C to -70°C for 24 months in lyophilized state;
- -70°C for 12 months under sterile conditions after reconstitution.
质量管理控制体系(QMS)
推荐产品
数据展示
电泳(SDS-PAGE)

Biotinylated Human TGF-Beta 1, Avitag on SDS-PAGE under reducing (R) and non-reducing (NR) conditions. The gel was stained with Coomassie Blue. The purity of the protein is greater than 95%.
活性(Bioactivity)-ELISA

Immobilized Human TGFBR2, Fc Tag (Cat. No. TG2-H5252) at 5 μg/mL (100 μL/well) can bind Biotinylated Human TGF-Beta 1, Avitag (Cat. No. TG1-H8217) with a linear range of 0.195-3 ng/mL (QC tested).
Protocol
Immobilized Biotinylated Human TGF-Beta 1, Avitag (Cat. No. TG1-H8217) at 1 μg/mL (100 μL/well) on streptavidin (Cat. No. STN-N5116) precoated (0.5 μg/well) plate can bind Human TGFBR2, Fc Tag (Cat. No. TG2-H5252) with a linear range of 0.3-10 ng/mL (Routinely tested).
Protocol
用户评价 发表评论



















