Human TAP1 & TAP2 Full Length Protein, Flag,His Tag&Flag,Twin-Strep Tag (Detergent)

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TA2-H55D3-20ug
¥7350.00
TA2-H55D3-100ug
¥27300.00
TA2-H55D3-500ug
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合计0件 产品金额¥ 0

产品详情

  • 分子别名(Synonym)

    TAP1 & TAP2

  • 表达区间及表达系统(Source)

    Human TAP1 & TAP2 Full Length Protein, Flag,His Tag&Flag,Twin-Strep Tag (TA2-H55D3) is expressed from human 293 cells (HEK293). It contains AA Ala 2 - Glu 748 & Arg 2 - Lys 686 (Accession # Q03518-1 & Q03519-1).

    Predicted N-terminus: Met & Asp

    Request for sequence
  • 蛋白结构(Molecular Characterization)

    TAP1 & TAP2 Structure

    Human TAP1 & TAP2 Full Length Protein, Flag,His Tag&Flag,Twin-Strep Tag, produced by co-expression of TAP1 and TAP2, has a calculated MW of 84.9 kDa (TAP1) and 81.1 kDa (TAP2). Subunit TAP1 is fused with a flag tag at the N-terminus and a polyhistidine tag at the C-terminus, and subunit TAP2 contains a flag tag at the N-terminus and a twin strep tag at the C-terminus. The protein migrates as 55 kDa and 65-70 kDa when calibrated against Star Ribbon Pre-stained Protein Marker under reducing (R) condition (SDS-PAGE).

  • 纯度(Purity)

    >90% as determined by SDS-PAGE.

  • 制剂(Formulation)

    This product is not suitable for cell based experiments due to cytotoxicity of DDM.
    DDM and CHS are INDISPENSABLE to keep membrane protein soluble and active, under no circumstances should you remove DDM and CHS.
    DDM/CHS buffer (DC-11) is sold separately and not included in protein, and please contact us if you need the buffer.
    If glycerol is not compatible with your application, remove glycerol just before the immediate experiment, and NEVER store glycerol-free protein solution.

    Supplied as 0.2 μm filtered solution in 50 mM HEPES, 150 mM NaCl, DDM, pH7.5, CHS with glycerol as protectant.

    Contact us for customized product form or formulation.

  • 运输(Shipping)

    This product is supplied and shipped with dry ice, please inquire the shipping cost.

  • 存储(Storage)

    Please avoid repeated freeze-thaw cycles.

    This product is stable after storage at:

    1. The product MUST be stored at -70°C or lower upon receipt;
    2. -70°C for 3 months under sterile conditions.
  • 质量管理控制体系(QMS)

    1. 质量管理体系(ISO, GMP)
    2. 质量优势
    3. 质控流程
  • *The DDM/CHS buffer (Cat. No. DC-11) is sold separately and not included in protein, you can follow this link for product information.

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  • 电泳(SDS-PAGE)

    TAP1 & TAP2 SDS-PAGE

    Human TAP1 & TAP2 Full Length Protein, Flag,His Tag&Flag,Twin-Strep Tag on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 90% (With Star Ribbon Pre-stained Protein Marker).

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背景介绍

The membrane-associated protein encoded by this gene is a member of the superfamily of ATP-binding cassette (ABC) transporters. ABC proteins transport various molecules across extra- and intra-cellular membranes. ABC genes are divided into seven distinct subfamilies (ABC1, MDR/TAP, MRP, ALD, OABP, GCN20, White). This protein is a member of the MDR/TAP subfamily. Members of the MDR/TAP subfamily are involved in multidrug resistance. The protein encoded by this gene is involved in the pumping of degraded cytosolic peptides across the endoplasmic reticulum into the membrane-bound compartment where class I molecules assemble. Mutations in this gene may be associated with ankylosing spondylitis, insulin-dependent diabetes mellitus, and celiac disease. Two transcript variants encoding different isoforms have been found for this gene.

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