Streptavidin Protein-Texas Red

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货号-规格
价格
Qty.
STN-NT113-100ug
¥630.00
STN-NT113-1mg (200ug X 5)
¥2520.00
合计0件 产品金额¥ 0

产品详情

  • 分子别名(Synonym)

    Streptavidin, SA

  • 表达区间及表达系统(Source)

    Streptavidin Protein-Texas Red (STN-NT113) is expressed from E. coli cells.

  • 蛋白结构(Molecular Characterization)

    This protein carries no "tag".

    The protein has a calculated MW of 13.8 kDa.

  • 偶联(Conjugate)

    Texas Red

    Excitation Wavelength: 586 nm

    Emission Wavelength: 603 nm

  • 标记(Labeling)

    The primary amines in the side chains of lysine residues and the N-terminus of the protein are conjugated with Texas Red using standard chemical labeling method. The residual Texas Red is removed by molecular sieve treatment during purification process.

  • 纯度(Purity)

    >95% as determined by SDS-PAGE.

  • 制剂(Formulation)

    Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.

    Contact us for customized product form or formulation.

  • 重构方法(Reconstitution)

    Please see Certificate of Analysis for specific instructions.

    For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

  • 存储(Storage)

    For long term storage, the product should be stored at lyophilized state at -20°C or lower.

    Please protect from light and avoid repeated freeze-thaw cycles.

    This product is stable after storage at:

    1. -20°C to -70°C for 12 months in lyophilized state;
    2. -70°C for 3 months under sterile conditions after reconstitution.
  • 质量管理控制体系(QMS)

    1. 质量管理体系(ISO, GMP)
    2. 质量优势
    3. 质控流程

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数据展示

  • 活性(Bioactivity)-FACS

     Streptavidin FACS

    5e5 of Anti-CD19 CAR-293 cells were stained with 100 μL of 20 ug/mL Biotinylated Human CD19 (20-291) Protein, Fc, Avitag™, premium grade (Cat. No. CD9-H82F6) and negative control protein respectively, washed and then followed with 1 μg/mL of Streptavidin Protein-Texas Red (Cat. No. STN-NT113) and analyzed with FACS. Texas Red signal was used to evaluate the binding activity (QC tested).

    Protocol

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背景介绍

The Streptavidin Protein Europium chelate is a universal tool for TR-FRET assays that can bind biotinylated molecules. This product uses high-purity streptavidin (SA) covalently conjugated with Eu³⁺ chelate, and it can be used in combination with other Acceptors directly or indirectly labeled with fluorescent dyes. When the Donor and Acceptor come into close proximity (within a distance of less than 10 nm), a FRET reaction occurs: the 620 nm signal emitted by the Donor upon excitation by a specific light source is received by the Acceptor, which then emits a 665 nm signal.

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