Biotinylated Human STEAP2 Full Length Protein, Flag,Avitag™ (Nanodisc-pro)

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Qty.
ST2-H85D3-20ug
¥7283.00
ST2-H85D3-100ug
¥12995.00
合计0件 产品金额¥ 0

产品详情

  • 分子别名(Synonym)

    STEAP2

  • 表达区间及表达系统(Source)

    Biotinylated Human STEAP2 Full Length Protein, Flag,Avitag (ST2-H85D3) is expressed from Baculovirus-Insect cells. It contains AA Glu 2 - Met 490 (Accession # Q8NFT2-1).

    Predicted N-terminus: Met

    Request for sequence
  • 蛋白结构(Molecular Characterization)

    STEAP2 Structure

    This protein carries a flag tag at the C-terminus, followed by an Avi tag (Avitag™).

    The protein has a calculated MW of 59.7 kDa.

    Nanodiscs are a new class of model membranes that are being used to solubilize and study a range of integral membrane proteins and membrane-associated proteins. The Nanodisc bilayer is bounded by an amphipathic scaffold coat that confers enhanced stability and a narrow particle size distribution.

    The nanodisc-pro is directly extracted from cell membrane in a water-soluble form using an amphipathic scaffold followed by affinity chromatography, and thus, it conserves a patch of native membrane around them.

  • 标记(Labeling)

    Biotinylation of this product is performed using Avitag™ technology. Briefly, the single lysine residue in the Avitag is enzymatically labeled with biotin.

  • 蛋白标记度(Protein Ratio)

    Passed as determined by the HABA assay / binding ELISA.

  • 纯度(Purity)

    >90% as determined by SDS-PAGE.

  • 制剂(Formulation)

    Supplied as 0.2 μm filtered solution in 20 mM HEPES, 150 mM NaCl, pH7.5 with glycerol as protectant.

    Contact us for customized product form or formulation.

  • 运输(Shipping)

    This product is supplied and shipped with dry ice, please inquire the shipping cost.

  • 存储(Storage)

    Please avoid repeated freeze-thaw cycles.

    This product is stable after storage at:

    1. The product MUST be stored at -70°C or lower upon receipt;
    2. -70°C for 3 months under sterile conditions.
  • 质量管理控制体系(QMS)

    1. 质量管理体系(ISO, GMP)
    2. 质量优势
    3. 质控流程
  • *The isotype control of empty/mock nanodisc (Cat. No. APO-H5143) is sold separately and not included in protein, you can follow the link for product information.

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  • 活性(Bioactivity)-ELISA

     STEAP2 ELISA

    Immobilized Biotinylated Human STEAP2 Full Length Protein, Flag,Avitag (Cat. No. ST2-H85D3) at 5 μg/mL (100 μL/well) on streptavidin (Cat. No. STN-N5116) precoated (0.5 μg/well) plate can bind Anti-STEAP2 Antibody with a linear range of 0.04-1.25 μg/mL (QC tested).

    Protocol

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背景介绍

This gene is a member of the STEAP family and encodes a multi-pass membrane protein that localizes to the Golgi complex, the plasma membrane, and the vesicular tubular structures in the cytosol. A highly similar protein in mouse has both ferrireductase and cupric reductase activity, and stimulates the cellular uptake of both iron and copper in vitro. Increased transcriptional expression of the human gene is associated with prostate cancer progression. Alternate transcriptional splice variants, encoding different isoforms, have been characterized.

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