SARS-CoV-2 Spike RBD Protein, His Tag (LP.8.1/Omicron) (MALS verified)

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价格
Qty.
SPD-S52He-100ug
¥3990.00
SPD-S52He-1mg (500ug X 2)
¥26700.00
合计0件 产品金额¥ 0

产品详情

  • 分子别名(Synonym)

    Spike, S protein RBD, Spike glycoprotein Receptor-binding domain, S glycoprotein RBD, Spike protein RBD

  • 表达区间及表达系统(Source)

    SARS-CoV-2 Spike RBD Protein, His Tag (LP.8.1/Omicron) (SPD-S52He) is expressed from human 293 cells (HEK293). It contains AA Arg 319 - Lys 537 (Accession # QHD43416.1 (I332V, G339H, R346T, K356T, S371F, S373P, S375F, T376A, R403K, D405N, R408S, K417N, N440K, V445R, G446S, N450D, L452W, L455S, F456L, N460K, S477N, T478K, N481K, V483del, E484K, F486P, Q493E, Q498R, N501Y, Y505H)).

    Predicted N-terminus: Arg 319

    Request for sequence
  • 蛋白结构(Molecular Characterization)

    This protein carries a polyhistidine tag at the C-terminus.

    The protein has a calculated MW of 26.6 kDa. The protein migrates as 38-43 kDa when calibrated against Star Ribbon Pre-stained Protein Marker under reducing (R) condition (SDS-PAGE) due to glycosylation.

  • 内毒素(Endotoxin)

    Less than 1.0 EU per μg by the LAL method / rFC method.

  • 纯度(Purity)

    >90% as determined by SDS-PAGE.

    >90% as determined by SEC-MALS.

  • 制剂(Formulation)

    Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.

    Contact us for customized product form or formulation.

  • 重构方法(Reconstitution)

    Please see Certificate of Analysis for specific instructions.

    For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

  • 存储(Storage)

    For long term storage, the product should be stored at lyophilized state at -20°C or lower.

    Please avoid repeated freeze-thaw cycles.

    This product is stable after storage at:

    1. -20°C to -70°C for 12 months in lyophilized state;
    2. -70°C for 3 months under sterile conditions after reconstitution.
  • 质量管理控制体系(QMS)

    1. 质量管理体系(ISO, GMP)
    2. 质量优势
    3. 质控流程

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数据展示

  • 电泳(SDS-PAGE)

    Spike RBD SDS-PAGE

    SARS-CoV-2 Spike RBD Protein, His Tag (LP.8.1/Omicron) on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 90% (With Star Ribbon Pre-stained Protein Marker).

  • SEC-MALS

    Spike RBD SEC-MALS

    The purity of SARS-CoV-2 Spike RBD Protein, His Tag (LP.8.1/Omicron) (Cat. No. SPD-S52He) is more than 90% and the molecular weight of this protein is around 30-45 kDa verified by SEC-MALS.

    Report
  • 活性(Bioactivity)-ELISA

     Spike RBD ELISA

    Immobilized Human ACE2, Fc Tag (Cat. No. AC2-H5257) at 5 μg/mL (100 μL/well) can bind SARS-CoV-2 Spike RBD Protein, His Tag (LP.8.1/Omicron) (Cat. No. SPD-S52He) with a linear range of 0.01-0.156 μg/mL (QC tested).

    Protocol
  •  Spike RBD ELISA

    Immobilized SARS-CoV-2 Spike RBD Protein, His Tag (LP.8.1/Omicron) (Cat. No. SPD-S52He) at 5 μg/mL (100 μL/well) can bind Human ACE2, Fc Tag (Cat. No. AC2-H5257) with a linear range of 0.4-25 ng/mL (Routinely tested).

    Protocol

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背景介绍

SARS‑CoV‑2 LP.8.1 is an Omicron sublineage first detected through genomic surveillance, carrying distinctive spike protein mutations linked to enhanced immune escape and potential increases in transmissibility. This variant remains under close monitoring by public health agencies for its impact on vaccine effectiveness, therapeutic antibody response, and spread dynamics, contributing valuable insight to ongoing COVID‑19 research and control strategies worldwide.

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