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MERS S protein RBD, His Tag

COADS MS
DS
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SPD-M52H6-100ug
¥4200.00
SPD-M52H6-1mg
¥27300.00
合计0件 产品金额¥ 0
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产品详情

  • 分子别名（Synonym）

    Spike,S protein RBD,Spike glycoprotein Receptor-binding domain,S glycoprotein RBD,Spike protein RBD

  • 表达区间及表达系统（Source）

    MERS S protein RBD, His Tag (SPD-M52H6) is expressed from human 293 cells (HEK293). It contains AA Glu 367 - Tyr 606 (Accession # K0BRG7-1).

    Predicted N-terminus: Glu 367

    Request for sequence

  • 蛋白结构（Molecular Characterization）

    Spike RBD Structure

    This protein carries a polyhistidine tag at the C-terminus.

    The protein has a calculated MW of 28.2 kDa. The protein migrates as 33-40 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

  • 内毒素（Endotoxin）

    Less than 1.0 EU per μg by the LAL method / rFC method.

  • 纯度（Purity）

    >90% as determined by SDS-PAGE.

  • 制剂（Formulation）

    Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.

    Contact us for customized product form or formulation.

  • 重构方法（Reconstitution）

    Please see Certificate of Analysis for specific instructions.

    For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

  • 存储（Storage）

    For long term storage, the product should be stored at lyophilized state at -20°C or lower.

    Please avoid repeated freeze-thaw cycles.

    This product is stable after storage at:

    1. -20°C to -70°C for 12 months in lyophilized state;
    2. -70°C for 3 months under sterile conditions after reconstitution.

  • 质量管理控制体系（QMS）

    1. 质量管理体系（ISO, GMP）
    2. 质量优势
    3. 质控流程

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数据展示

  • 电泳（SDS-PAGE）

    Spike RBD SDS-PAGE

    MERS S protein RBD, His Tag on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 90%.

  • 活性（Bioactivity）-ELISA

    Spike RBD ELISA

    Immobilized Human DPPIV, Fc Tag (Cat. No. DP4-H5266) at 5 μg/mL (100 μL/well) can bind MERS S protein RBD, His Tag (Cat. No. SPD-M52H6) with a linear range of 0.6-5 ng/mL (QC tested).

    Protocol

  • Spike RBD ELISA

    Immobilized MERS S protein RBD, His Tag (Cat. No. SPD-M52H6) at 5 μg/mL (100 μL/well) can bind Monoclonal Anti-SARS-CoV-S protein RBD Antibody, Human IgG1 with a linear range of 0.313-2.5 μg/mL (Routinely tested).

    Protocol

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背景介绍

The MERS or Middle East Respiratory Syndrome Coronavirus, is a member of the coronavirus family and is known to cause severe respiratory illness in humans. The MERS protein is a type I transmembrane protein that plays a vital role in the virus's ability to infect host cells. It is composed of three main domains: the extracellular domain, the transmembrane domain, and the cytoplasmic domain. The extracellular domain, further divided into S1 and S2 subunits, is responsible for receptor recognition and binding. The S1 subunit contains the Receptor Binding Domain (RBD), which is crucial for the virus's attachment to and entry into host cells. The RBD specifically interacts with the host cell receptor, facilitating the initial step of infection. The S2 subunit, on the other hand, is responsible for membrane fusion. It contains a fusion peptide that inserts into the host cell membrane, initiating the fusion process. This fusion allows the virus's genetic material to be released into the host cell, initiating the infection process.

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