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分子别名（Synonym）
Spike, S protein, Spike glycoprotein, S glycoprotein
表达区间及表达系统（Source）
SARS-CoV-2 Spike Trimer Protein, His Tag (NB.1.8.1) (SPN-S522b) is expressed from human 293 cells (HEK293). It contains AA Val 16 - Pro 1213 (Accession # QHD43416.1 (T19I, R21T, T22N, A27S, S50L, F59S, V127F, G142D, F157S, R158G, G184S, L212I, V213G, L216F, H245N, A264D, I332V, G339H, K356T, S371F, S373P, S375F, T376A, R403K, D405N, R408S, K417N, A435S, N440K, V445H, G446S, N450D, L452W, L455S, F456L, N460K, S477N, T478I, N481K, E484K, F486P, Q493E, Q498R, N501Y, Y505H, E554K, A570V, D614G, P621S, H655Y, N679K, P681R, N764K, F817P, A892P, A899P, A942P, K986P, V987P, D796Y, S939F, Q954F, N969K, P1143L)). The recombinant protein is expressed from human 293 cells (HEK293) with T4 fibritin trimerization motif and a polyhistidine tag at the C-terminus. Proline substitutions (F817P, A892P, A899P, A942P, K986P, V987P) are introduced to stabilize the trimeric prefusion state of SARS-CoV-2 S protein.
Predicted N-terminus: Val 16
Request for sequence蛋白结构（Molecular Characterization）
This protein carries a polyhistidine tag at the C-terminus.
The protein has a calculated MW of 138.6 kDa. The protein migrates as 80-90 kDa and 100-110 kDa when calibrated against Star Ribbon Pre-stained Protein Marker under reducing (R) condition (SDS-PAGE).
The protein is designed as a trimer.
内毒素（Endotoxin）
Less than 1.0 EU per μg by the LAL method / rFC method.
纯度（Purity）
>90% as determined by SDS-PAGE.
制剂（Formulation）
Lyophilized from 0.22 μm filtered solution in 0.1 M Sodium citrate with trehalose as protectant.
Contact us for customized product form or formulation.
重构方法（Reconstitution）
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
存储（Storage）
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
- -20°C to -70°C for 12 months in lyophilized state;
- -70°C for 3 months under sterile conditions after reconstitution.
质量管理控制体系（QMS）
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数据展示
电泳（SDS-PAGE）
SARS-CoV-2 Spike Trimer Protein, His Tag (NB.1.8.1) on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 90% (With Star Ribbon Pre-stained Protein Marker).
SEC-MALS
The purity of SARS-CoV-2 Spike Trimer Protein, His Tag (NB.1.8.1) (Cat. No. SPN-S522b) is more than 85% and the molecular weight of this protein is around 510-560 kDa verified by SEC-MALS.
Report
活性（Bioactivity）-ELISA
Immobilized Human ACE2, Fc Tag (Cat. No. AC2-H5257) at 5 μg/mL (100 μL/well) can bind SARS-CoV-2 Spike Trimer Protein, His Tag (NB.1.8.1) (Cat. No. SPN-S522b) with a linear range of 0.5-31 ng/mL (QC tested).
Protocol
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