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分子别名（Synonym）
Spike, S protein, Spike glycoprotein, S glycoprotein
表达区间及表达系统（Source）
SARS coronavirus Sino1-11 Spike Trimer Protein, His Tag (SPN-S5224) is expressed from human 293 cells (HEK293). It contains AA Ser 14 - Pro 1195 (Accession # AY485277.1 (R667A, KV968-969PP)).
Predicted N-terminus: Ser 14
Request for sequence蛋白结构（Molecular Characterization）
Other Tags and Version Biotin & Other Labeled Version
This protein carries a polyhistidine tag at the C-terminus.
The protein has a calculated MW of 136.2 kDa. The protein migrates as 150-180 kDa when calibrated against Star Ribbon Pre-stained Protein Marker under reducing (R) condition (SDS-PAGE) due to glycosylation.
纯度（Purity）
>95% as determined by SDS-PAGE.
>90% as determined by SEC-MALS.
制剂（Formulation）
Lyophilized from 0.22 μm filtered solution in 0.1 M Sodium citrate, pH5.5 with trehalose as protectant.
Contact us for customized product form or formulation.
重构方法（Reconstitution）
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
存储（Storage）
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
- -20°C to -70°C for 12 months in lyophilized state;
- -70°C for 3 months under sterile conditions after reconstitution.
质量管理控制体系（QMS）
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数据展示
电泳（SDS-PAGE）
SARS coronavirus Sino1-11 Spike Trimer Protein, His Tag on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 95% (With Star Ribbon Pre-stained Protein Marker).
SEC-MALS
The purity of SARS coronavirus Sino1-11 Spike Trimer Protein, His Tag (Cat. No. SPN-S5224) is more than 90% and the molecular weight of this protein is around 520-600 kDa verified by SEC-MALS.
Report
活性（Bioactivity）-ELISA
Immobilized Human ACE2, Fc Tag (Cat. No. AC2-H5257) at 5 μg/mL (100 μL/well) can bind SARS coronavirus Sino1-11 Spike Trimer Protein, His Tag (Cat. No. SPN-S5224) with a linear range of 0.001-0.063 μg/mL (QC tested).
Protocol
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