Pangolin coronavirus Spike NTD Protein, His Tag (MALS verified)

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SPD-P52H3-100ug
¥3360.00
SPD-P52H3-1mg
¥21840.00
合计0件 产品金额¥ 0

产品详情

  • 分子别名(Synonym)

    S1 protein NTD, Spike protein S1 NTD, BetaCoV S1-NTD

  • 表达区间及表达系统(Source)

    Pangolin coronavirus Spike NTD Protein, His Tag (SPD-P52H3) is expressed from human 293 cells (HEK293). It contains AA Ser 13 - Leu 303 (Accession # QVT76606.1).

    Predicted N-terminus: Ser 13

    Request for sequence
  • 蛋白结构(Molecular Characterization)

    Spike NTD Structure

    Other Tags and Version Biotin & Other Labeled Version

    This protein carries a polyhistidine tag at the C-terminus.

    The protein has a calculated MW of 35.1 kDa. The protein migrates as 43-58 kDa when calibrated against Star Ribbon Pre-stained Protein Marker under reducing (R) condition (SDS-PAGE) due to glycosylation.

  • 纯度(Purity)

    >95% as determined by SDS-PAGE.

    >90% as determined by SEC-MALS.

  • 制剂(Formulation)

    Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.

    Contact us for customized product form or formulation.

  • 重构方法(Reconstitution)

    Please see Certificate of Analysis for specific instructions.

    For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

  • 存储(Storage)

    For long term storage, the product should be stored at lyophilized state at -20°C or lower.

    Please avoid repeated freeze-thaw cycles.

    This product is stable after storage at:

    1. -20°C to -70°C for 12 months in lyophilized state;
    2. -70°C for 3 months under sterile conditions after reconstitution.
  • 质量管理控制体系(QMS)

    1. 质量管理体系(ISO, GMP)
    2. 质量优势
    3. 质控流程

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数据展示

  • 电泳(SDS-PAGE)

    Spike NTD SDS-PAGE

    Pangolin coronavirus Spike NTD Protein, His Tag on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 95% (With Star Ribbon Pre-stained Protein Marker).

  • SEC-MALS

    Spike NTD SEC-MALS

    The purity of Pangolin coronavirus Spike NTD Protein, His Tag (Cat. No. SPD-P52H3) is more than 90% and the molecular weight of this protein is around 45-65 kDa verified by SEC-MALS.

    Report
  • 活性(Bioactivity)-ELISA

     Spike NTD ELISA

    Immobilized Pangolin coronavirus Spike NTD Protein, His Tag (Cat. No. SPD-P52H3) at 1 μg/mL (100 μL/well) can bind Anti-SARS-CoV-2 Spike NTD Antibody, Chimeric mAb, Human IgG1 (AM121) (Cat. No. SPD-M121) with a linear range of 0.06-1 ng/mL (QC tested).

    Protocol

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背景介绍

The spike protein is a large type I transmembrane protein containing two subunits, S1 and S2. S1 mainly contains a receptor binding domain (RBD), which is responsible for recognizing the cell surface receptor. S2 contains basic elements needed for the membrane fusion.The S protein plays key parts in the induction of neutralizing-antibody and T-cell responses, as well as protective immunity.

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