Biotinylated Human SIRP gamma / CD172g Protein, His,Avitag™ (MALS verified)

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SIG-H82E3-25ug
¥3675.00
SIG-H82E3-200ug
¥14490.00
合计0件 产品金额¥ 0

产品详情

  • 分子别名(Synonym)

    SIRPG,CD172g,SIRPB2,SIRP-gamma,SIRP-b2,SIRP-beta-2

  • 表达区间及表达系统(Source)

    Biotinylated Human SIRP gamma, His,Avitag (SIG-H82E3) is expressed from human 293 cells (HEK293). It contains AA Glu 29 - Ser 364 (Accession # AAH64532.1).

    Predicted N-terminus: Glu 29

    Request for sequence
  • 蛋白结构(Molecular Characterization)

    SIRP gamma Structure

    This protein carries a polyhistidine tag at the C-terminus, followed by an Avi tag (Avitag™).

    The protein has a calculated MW of 40.7 kDa. The protein migrates as 48-55 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

  • 标记(Labeling)

    Biotinylation of this product is performed using Avitag™ technology. Briefly, the single lysine residue in the Avitag is enzymatically labeled with biotin.

  • 蛋白标记度(Protein Ratio)

    Passed as determined by the HABA assay / binding ELISA.

  • 纯度(Purity)

    >95% as determined by SDS-PAGE.

    >90% as determined by SEC-MALS.

  • 制剂(Formulation)

    Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.

    Contact us for customized product form or formulation.

  • 重构方法(Reconstitution)

    Please see Certificate of Analysis for specific instructions.

    For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

  • 存储(Storage)

    For long term storage, the product should be stored at lyophilized state at -20°C or lower.

    Please avoid repeated freeze-thaw cycles.

    This product is stable after storage at:

    1. -20°C to -70°C for 12 months in lyophilized state;
    2. -70°C for 3 months under sterile conditions after reconstitution.
  • 质量管理控制体系(QMS)

    1. 质量管理体系(ISO, GMP)
    2. 质量优势
    3. 质控流程

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数据展示

  • 电泳(SDS-PAGE)

    SIRP gamma SDS-PAGE

    Biotinylated Human SIRP gamma, His,Avitag on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 95%.

  • SEC-MALS

    SIRP gamma SEC-MALS

    The purity of Biotinylated Human SIRP gamma, His,Avitag (Cat. No. SIG-H82E3) is more than 90% and the molecular weight of this protein is around 45-57 kDa verified by SEC-MALS.

    Report
  • 活性(Bioactivity)-ELISA

     SIRP gamma ELISA

    Immobilized Biotinylated Human SIRP gamma, His,Avitag (Cat. No. SIG-H82E3) at 1 μg/mL (100 μL/well) on streptavidin (Cat. No. STN-N5116) precoated (0.5 μg/well) plate can bind Human CD47, Mouse IgG2a Fc Tag (Cat. No. CD7-H52A5) with a linear range of 4-31 ng/mL (QC tested).

    Protocol

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背景介绍

Signal-regulatory protein gamma (SIRPG or SIRP-gamma) is also known as CD172 antigen-like family member B (CD172g), Signal-regulatory protein beta-2 (SIRPB2), is a single-pass type I membrane protein. SIRPG is expressed on CD4+ T-cells, CD8+ T-cells, CD56-bright natural killer (NK) cells, CD20+ cells, and all activated NK cells. SIRPG interacts with CD47. On binding with CD47, CD172g mediates cell-cell adhesion.

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