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分子别名（Synonym）
SHPS1,SIRPA,CD172A,BIT,MFR,MYD1,P84,PTPNS1
表达区间及表达系统（Source）
Rat SIRP alpha, Fc Tag (SIA-R5255) is expressed from human 293 cells (HEK293). It contains AA Lys 32 - Asn 373 (Accession # BAA20368.1).
Predicted N-terminus: Lys 32
Request for sequence蛋白结构（Molecular Characterization）
This protein carries a human IgG1 Fc tag at the C-terminus.
The protein has a calculated MW of 64.0 kDa. The protein migrates as 100-116 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.
内毒素（Endotoxin）
Less than 1.0 EU per μg by the LAL method / rFC method.
纯度（Purity）
>95% as determined by SDS-PAGE.
制剂（Formulation）
Lyophilized from 0.22 μm filtered solution in 50 mM Tris, 100 mM Glycine, 25 mM Arginine, 150 mM NaCl, pH7.5 with trehalose as protectant.
Contact us for customized product form or formulation.
重构方法（Reconstitution）
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
存储（Storage）
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
- -20°C to -70°C for 12 months in lyophilized state;
- -70°C for 3 months under sterile conditions after reconstitution.
质量管理控制体系（QMS）
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数据展示
电泳（SDS-PAGE）
Rat SIRP alpha, Fc Tag on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 95%.
SEC-MALS
The purity of Rat SIRP alpha, Fc Tag (Cat. No. SIA-R5255) is more than 85% and the molecular weight of this protein is around 150-180 kDa verified by SEC-MALS.
Report
活性（Bioactivity）-ELISA
Immobilized Rat SIRP alpha, Fc Tag (Cat. No. SIA-R5255) at 5 μg/mL (100 μL/well) can bind Mouse CD47, Mouse IgG2a Fc Tag, low endotoxin (Cat. No. CD7-M525a) with a linear range of 1-20 ng/mL (QC tested).
Protocol
Immobilized Mouse CD47, Mouse IgG2a Fc Tag, low endotoxin (Cat. No. CD7-M525a) at 5 μg/mL (100 μL/well) can bind Rat SIRP alpha, Fc Tag (Cat. No. SIA-R5255) with a linear range of 2-39 ng/mL (Routinely tested).
Protocol
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