关注公众号
收藏产品 产品详情
分子别名（Synonym）
SHPS1, SIRPA, CD172A, BIT, MFR, MYD1, P84, PTPNS1
表达区间及表达系统（Source）
Human SIRP alpha, Mouse IgG1 Fc Tag (SIA-H52A8) is expressed from human 293 cells (HEK293). It contains AA Glu 31 - Arg 370 (Accession # NP_001035111).
Predicted N-terminus: Glu 31
Request for sequence蛋白结构（Molecular Characterization）
This protein carries a mouse IgG1 Fc tag at the C-terminus.
The protein has a calculated MW of 63.0 kDa. The protein migrates as 70-105 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.
内毒素（Endotoxin）
Less than 1.0 EU per μg by the LAL method / rFC method.
纯度（Purity）
>95% as determined by SDS-PAGE.
>90% as determined by SEC-MALS.
制剂（Formulation）
Lyophilized from 0.22 μm filtered solution in 50 mM Tris, 100 mM Glycine, pH7.5 with trehalose as protectant.
Contact us for customized product form or formulation.
重构方法（Reconstitution）
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
存储（Storage）
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
- -20°C to -70°C for 12 months in lyophilized state;
- -70°C for 3 months under sterile conditions after reconstitution.
质量管理控制体系（QMS）
推荐产品
数据展示
电泳（SDS-PAGE）
Human SIRP alpha, Mouse IgG1 Fc Tag on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 95%.
SEC-MALS
The purity of Human SIRP alpha, Mouse IgG1 Fc Tag (Cat. No. SIA-H52A8) is more than 90% and the molecular weight of this protein is around 140-165 kDa verified by SEC-MALS.
Report
活性（Bioactivity）-ELISA
Immobilized Human CD47, His Tag (Cat. No. CD7-H5227) at 2 μg/mL (100 μL/well) can bind Human SIRP alpha, Mouse IgG1 Fc Tag (HPLC-verified) (Cat. No. SIA-H52A8) with a linear range of 4-31 ng/mL (QC tested).
Protocol
Serial dilutions of Anti-Human CD47 Neutralizing Antibody were added into Human SIRP alpha, Mouse IgG1 Fc Tag (Cat. No. SIA-H52A8): Biotinylated Human CD47, Fc,Avitag (Cat. No. CD7-H82F6) binding reactions. The half maximal inhibitory concentration (IC50) is 0.2006 μg/mL (Routinely tested).
Protocol
活性（Bioactivity）-FACS
FACS assay shows that Human SIRP alpha, Mouse IgG1 Fc Tag (Cat. No. SIA-H52A8) can bind to Jurkat cell expressing CD47. The concentration of SIRP alpha used is 0.3 μg/mL (Routinely tested).
Protocol
FACS analysis shows that the binding of Human SIRP alpha, Mouse IgG1 Fc Tag (Cat. No. SIA-H52A8) to Jurkat expressing CD47 was inhibited by increasing concentration of neutralizing anti-CD47 antibody. The concentration of SIRP alpha used is 0.3 μg/mL. IC50=0.1318 μg/mL (Routinely tested).
Protocol
用户评价 发表评论
药物研发进展 
联系我们 
项目合作 