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分子别名（Synonym）
CD33,SIGLEC3,gp67
表达区间及表达系统（Source）
Rat Siglec-3, His Tag (CD3-R52H4) is expressed from human 293 cells (HEK293). It contains AA Asp 18 - Gln 240 (Accession # F1LTZ3-1).
Predicted N-terminus: Asp 18
Request for sequence蛋白结构（Molecular Characterization）
This protein carries a polyhistidine tag at the C-terminus.
The protein has a calculated MW of 26.7 kDa. The protein migrates as 37-45 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.
内毒素（Endotoxin）
Less than 1.0 EU per μg by the LAL method / rFC method.
纯度（Purity）
>95% as determined by SDS-PAGE.
>90% as determined by SEC-MALS.
制剂（Formulation）
Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.
Contact us for customized product form or formulation.
重构方法（Reconstitution）
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
存储（Storage）
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
- -20°C to -70°C for 12 months in lyophilized state;
- -70°C for 3 months under sterile conditions after reconstitution.
质量管理控制体系（QMS）
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数据展示
电泳（SDS-PAGE）
Rat Siglec-3, His Tag on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 95%.
SEC-MALS
The purity of Rat Siglec-3, His Tag (Cat. No. CD3-R52H4) is more than 90% and the molecular weight of this protein is around 32-47 kDa verified by SEC-MALS.
Report
活性（Bioactivity）-ELISA
Immobilized Rat Siglec-3, His Tag (Cat. No. CD3-R52H4) at 1 μg/mL (100 μL/well) can bind Anti-CD33 antibody, Mouse IgG1 with a linear range of 0.2-16 ng/mL (QC tested).
Protocol
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