FITC-Labeled Human Siglec-3 / CD33 Protein, Fc Tag

分子别名（Synonym）

CD33, SIGLEC3, gp67

表达区间及表达系统（Source）

FITC-Labeled Human Siglec-3, Fc Tag (CD3-HF256) is expressed from human 293 cells (HEK293). It contains AA Asp 18 - His 259 (Accession # AAH28152.1). It is the FITC labeled form of Human Siglec-3, Fc Tag (Cat. No. CD3-H5257).

Predicted N-terminus: Asp 18

蛋白结构（Molecular Characterization）

This protein carries a human IgG1 Fc tag at the C-terminus.

The protein has a calculated MW of 52.9 kDa. The protein migrates as 70-90 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

偶联（Conjugate）

FITC

Excitation source: 488 nm spectral line, argon-ion laser

Excitation Wavelength: 488 nm

Emission Wavelength: 535 nm

标记（Labeling）

The primary amines in the side chains of lysine residues and the N-terminus of the protein are conjugated with FITC using standard chemical labeling method. The residual FITC is removed by molecular sieve treatment during purification process.

蛋白标记度（Protein Ratio）

The FITC to protein molar ratio is 2-4.

纯度（Purity）

>90% as determined by SDS-PAGE.

制剂（Formulation）

Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.

Contact us for customized product form or formulation.

重构方法（Reconstitution）

Please see Certificate of Analysis for specific instructions.

For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

存储（Storage）

For long term storage, the product should be stored at lyophilized state at -20°C or lower.

Please protect from light and avoid repeated freeze-thaw cycles.

This product is stable after storage at:

1. -20°C to -70°C for 12 months in lyophilized state;
2. -70°C for 3 months under sterile conditions after reconstitution.

质量管理控制体系（QMS）

1. 质量管理体系（ISO, GMP）
2. 质量优势
3. 质控流程