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分子别名（Synonym）
CD33 antigen-like 3, SIGLEC-15, CD33L3, sialic acid-binding Ig-like lectin 15, Siglec15, Siglec-15
表达区间及表达系统（Source）
Rat Siglec-15 Protein, His Tag (SG5-R52H3) is expressed from human 293 cells (HEK293). It contains AA Arg 24 - Pro 257 (Accession # D3ZL05).
Predicted N-terminus: Arg 24
Request for sequence蛋白结构（Molecular Characterization）
Other Tags and Version Biotin & Other Labeled Version
This protein carries a polyhistidine tag at the C-terminus.
The protein has a calculated MW of 27.4 kDa. The protein migrates as 32-37 kDa when calibrated against Star Ribbon Pre-stained Protein Marker under reducing (R) condition (SDS-PAGE) due to glycosylation.
The protein is designed as a dimer.
内毒素（Endotoxin）
Less than 1.0 EU per μg by the LAL method / rFC method.
纯度（Purity）
>90% as determined by SDS-PAGE.
制剂（Formulation）
Lyophilized from 0.22 μm filtered solution in PBS, 0.5 M Arginine, pH7.4 with trehalose as protectant.
Contact us for customized product form or formulation.
重构方法（Reconstitution）
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
存储（Storage）
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
- -20°C to -70°C for 12 months in lyophilized state;
- -70°C for 3 months under sterile conditions after reconstitution.
质量管理控制体系（QMS）
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数据展示
电泳（SDS-PAGE）
Rat Siglec-15 Protein, His Tag on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 90% (With Star Ribbon Pre-stained Protein Marker).
SEC-MALS
The purity of Rat Siglec-15 Protein, His Tag (Cat. No. SG5-R52H3) is more than 85% and the molecular weight of this protein is around 58-73 kDa verified by SEC-MALS.
Report
活性（Bioactivity）-ELISA
Immobilized Rat Siglec-15 Protein, His Tag (Cat. No. SG5-R52H3) at 1 μg/mL (100 μL/well) can bind Mouse Human chimeric mAb (5G12, Human IgG1) with a linear range of 0.06-2 ng/mL (QC tested).
Protocol
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