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分子别名（Synonym）
BSRPC
表达区间及表达系统（Source）
Human SEZ6 Protein, Fc Tag (SE6-H5253) is expressed from human 293 cells (HEK293). It contains AA Leu 20 - Ala 414 (Accession # Q53EL9-1).
Predicted N-terminus: Leu 20
Request for sequence蛋白结构（Molecular Characterization）
This protein carries a human IgG1 Fc tag at the C-terminus.
The protein has a calculated MW of 68.4 kDa. The protein migrates as 85-130 kDa when calibrated against Star Ribbon Pre-stained Protein Marker under reducing (R) condition (SDS-PAGE) due to glycosylation.
内毒素（Endotoxin）
Less than 1.0 EU per μg by the LAL method / rFC method.
纯度（Purity）
>90% as determined by SDS-PAGE.
制剂（Formulation）
Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.
Contact us for customized product form or formulation.
重构方法（Reconstitution）
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
存储（Storage）
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
- -20°C to -70°C for 12 months in lyophilized state;
- -70°C for 3 months under sterile conditions after reconstitution.
质量管理控制体系（QMS）
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数据展示
电泳（SDS-PAGE）
Human SEZ6 Protein, Fc Tag on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 90% (With Star Ribbon Pre-stained Protein Marker).
SEC-MALS
The purity of Human SEZ6 Protein, Fc Tag (Cat. No. SE6-H5253) is more than 85% and the molecular weight of this protein is around 160-195 kDa verified by SEC-MALS.
Report
活性（Bioactivity）-ELISA
Immobilized Human SEZ6 Protein, Fc Tag (Cat. No. SE6-H5253) at 1 μg/mL (100 μL/well) can bind Anti-SEZ6 Antibody (SC17) with a linear range of 0.2-6 ng/mL (QC tested).
Protocol
活性（Bioactivity）-SPR
Turmetabart immobilized on CM5 Chip can bind Human SEZ6 Protein, Fc Tag (Cat. No. SE6-H5253) with an affinity constant of 1.11 nM as determined in a SPR assay (Biacore 8K) (Routinely tested).
Protocol
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