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分子别名（Synonym）
Semaphorin 3A, (semaphorin) 3A, coll-1, collapsin 1, Hsema-I, Hsema-III, MGC133243, sema domain, immunoglobulin domain (Ig), short basic domain, secreted, Sema III, SEMA1, Sema3A, SEMAD, SEMAIII, SEMAL, Semaphorin 3A, semaphorin D, Semaphorin III, semaphorin L, semaphorin-3A, SemD, SEMA3A
表达区间及表达系统（Source）
Human Semaphorin 3A, Fc Tag (SEA-H5259) is expressed from human 293 cells (HEK293). It contains AA Lys 26 - Val 771 (Accession # Q14563-1 (R551A, R555A, R730A, K731A, R733A, R734A, R757A, R760A)).
Predicted N-terminus: Pro
Request for sequence蛋白结构（Molecular Characterization）
This protein carries a human IgG1 Fc tag at the N-terminus.
The protein has a calculated MW of 111.8 kDa. The protein migrates as 120-135 kDa when calibrated against Star Ribbon Pre-stained Protein Marker under reducing (R) condition (SDS-PAGE) due to glycosylation.
内毒素（Endotoxin）
Less than 1.0 EU per μg by the LAL method / rFC method.
纯度（Purity）
>95% as determined by SDS-PAGE.
>95% as determined by SEC-MALS.
制剂（Formulation）
Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.
Contact us for customized product form or formulation.
重构方法（Reconstitution）
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
存储（Storage）
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
- -20°C to -70°C for 12 months in lyophilized state;
- -70°C for 3 months under sterile conditions after reconstitution.
质量管理控制体系（QMS）
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数据展示
电泳（SDS-PAGE）
Human Semaphorin 3A, Fc Tag on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 95% (With Star Ribbon Pre-stained Protein Marker).
SEC-MALS
The purity of Human Semaphorin 3A, Fc Tag (Cat. No. SEA-H5259) is more than 95% and the molecular weight of this protein is around 220-269 kDa verified by SEC-MALS.
Report
活性（Bioactivity）-ELISA
Immobilized Human Neuropilin-1, His Tag (Cat. No. NR1-H5228) at 5 μg/mL (100 μL/well) can bind Human Semaphorin 3A, Fc Tag (Cat. No. SEA-H5259) with a linear range of 2-78 ng/mL (QC tested).
Protocol
Immobilized Biotinylated Human Neuropilin-1, His,Avitag (Cat. No. NR1-H82E3) at 1 μg/mL (100 μL/well) on streptavidin (Cat. No. STN-N5116) precoated (0.5 μg/well) plate can bind Human Semaphorin 3A, Fc Tag (Cat. No. SEA-H5259) with a linear range of 0.6-20 ng/mL (QC tested).
Protocol
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