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分子别名（Synonym）
PTK7, CCK4, CCK-4, Tyrosine-protein kinase-like 7, Protein-tyrosine kinase 7, Protein-tyrosine kinase 7
表达区间及表达系统（Source）
Biotinylated Human PTK7 protein, Fc,Avitag (PT7-H82F5) is expressed from human 293 cells (HEK293). It contains AA Ala 31 - Thr 704 (Accession # Q13308-1).
Predicted N-terminus: Ala 31
Request for sequence蛋白结构（Molecular Characterization）
This protein carries a human IgG1 Fc tag at the C-terminus, followed by an Avi tag (Avitag™).
The protein has a calculated MW of 102.8 kDa. The protein migrates as 125-145 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.
标记（Labeling）
Biotinylation of this product is performed using Avitag™ technology. Briefly, the single lysine residue in the Avitag is enzymatically labeled with biotin.
蛋白标记度（Protein Ratio）
Passed as determined by the HABA assay / binding ELISA.
纯度（Purity）
>90% as determined by SDS-PAGE.
>90% as determined by SEC-MALS.
制剂（Formulation）
Lyophilized from 0.22 μm filtered solution in 50 mM Tris, 100 mM Glycine, 25 mM Arginine, 150 mM NaCl, pH7.5 with trehalose as protectant.
Contact us for customized product form or formulation.
重构方法（Reconstitution）
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
存储（Storage）
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
- -20°C to -70°C for 12 months in lyophilized state;
- -70°C for 3 months under sterile conditions after reconstitution.
质量管理控制体系（QMS）
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数据展示
电泳（SDS-PAGE）
Biotinylated Human PTK7 protein, Fc,Avitag on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 90%.
SEC-MALS
The purity of Biotinylated Human PTK7 protein, Fc,Avitag (Cat. No. PT7-H82F5) is more than 90% and the molecular weight of this protein is around 235-265 kDa verified by SEC-MALS.
Report
活性（Bioactivity）-ELISA
Immobilized Biotinylated Human PTK7 protein, Fc,Avitag (Cat. No. PT7-H82F5) at 1 μg/mL (100 μL/well) on streptavidin (Cat. No. STN-N5116) precoated (0.5 μg/well) plate can bind Cofetuzumab Pelidotin with a linear range of 0.06-4 ng/mL (QC tested).
Protocol
活性（Bioactivity）-SPR
Biotinylated Human PTK7 protein, Fc,Avitag (Cat. No. PT7-H82F5) captured on Biotin CAP-Series S Sensor Chip can bind Cofetuzumab with an affinity constant of 58.2 pM as determined in a SPR assay (Biacore 8K) (Routinely tested).
Protocol
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