产品详情
分子别名(Synonym)
Prostatic Acid Phosphatase, ACPP, ACP-3, PAP, 5'-NT, TMPase, TM-PAP
表达区间及表达系统(Source)
Biotinylated Human Prostatic Acid Phosphatase (33-382) Protein, His,Avitag (PRE-H82E9) is expressed from human 293 cells (HEK293). It contains AA Lys 33 - Lys 382 (Accession # P15309-2).
Predicted N-terminus: Lys 33
Request for sequence蛋白结构(Molecular Characterization)

This protein carries a polyhistidine tag at the C-terminus, followed by an Avi tag (Avitag™).
The protein has a calculated MW of 44.2 kDa. The protein migrates as 50-55 kDa when calibrated against Star Ribbon Pre-stained Protein Marker under reducing (R) condition (SDS-PAGE) due to glycosylation.
The protein is designed as a dimer.
标记(Labeling)
Biotinylation of this product is performed using Avitag™ technology. Briefly, the single lysine residue in the Avitag is enzymatically labeled with biotin.
蛋白标记度(Protein Ratio)
Passed as determined by the HABA assay / binding ELISA.
内毒素(Endotoxin)
Less than 1.0 EU per μg by the LAL method / rFC method.
纯度(Purity)
>90% as determined by SDS-PAGE.
>95% as determined by SEC-MALS.
制剂(Formulation)
Supplied as 0.2 μm filtered solution in 100 mM NaAC, pH5.0 with glycerol as protectant.
Contact us for customized product form or formulation.
运输(Shipping)
This product is supplied and shipped with dry ice, please inquire the shipping cost.
存储(Storage)
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
- The product MUST be stored at -70°C or lower upon receipt;
- -70°C for 3 months under sterile conditions.
质量管理控制体系(QMS)
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数据展示
电泳(SDS-PAGE)

Biotinylated Human Prostatic Acid Phosphatase (33-382) Protein, His,Avitag on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 90% (With Star Ribbon Pre-stained Protein Marker).
SEC-MALS

The purity of Biotinylated Human Prostatic Acid Phosphatase (33-382) Protein, His,Avitag (Cat. No. PRE-H82E9) is more than 95% and the molecular weight of this protein is around 88-118 kDa verified by SEC-MALS.
Report
活性(Bioactivity)
Measured by its ability to cleave a substrate, p-Nitrophenyl phosphate (pNPP). The specific activity is >65,000 pmol/min/ug (QC tested).
Protocol
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