Biotinylated Human Prostatic Acid Phosphatase / ACPP (33-382) Protein, His,Avitag™ (active enzyme, MALS verified)

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货号-规格
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Qty.
PRE-H82E9-25ug
¥3800.00
PRE-H82E9-200ug
¥15700.00
合计0件 产品金额¥ 0

产品详情

  • 分子别名(Synonym)

    Prostatic Acid Phosphatase, ACPP, ACP-3, PAP, 5'-NT, TMPase, TM-PAP

  • 表达区间及表达系统(Source)

    Biotinylated Human Prostatic Acid Phosphatase (33-382) Protein, His,Avitag (PRE-H82E9) is expressed from human 293 cells (HEK293). It contains AA Lys 33 - Lys 382 (Accession # P15309-2).

    Predicted N-terminus: Lys 33

    Request for sequence
  • 蛋白结构(Molecular Characterization)

    Prostatic Acid Phosphatase Structure

    This protein carries a polyhistidine tag at the C-terminus, followed by an Avi tag (Avitag™).

    The protein has a calculated MW of 44.2 kDa. The protein migrates as 50-55 kDa when calibrated against Star Ribbon Pre-stained Protein Marker under reducing (R) condition (SDS-PAGE) due to glycosylation.

    The protein is designed as a dimer.

  • 标记(Labeling)

    Biotinylation of this product is performed using Avitag™ technology. Briefly, the single lysine residue in the Avitag is enzymatically labeled with biotin.

  • 蛋白标记度(Protein Ratio)

    Passed as determined by the HABA assay / binding ELISA.

  • 内毒素(Endotoxin)

    Less than 1.0 EU per μg by the LAL method / rFC method.

  • 纯度(Purity)

    >90% as determined by SDS-PAGE.

    >95% as determined by SEC-MALS.

  • 制剂(Formulation)

    Supplied as 0.2 μm filtered solution in 100 mM NaAC, pH5.0 with glycerol as protectant.

    Contact us for customized product form or formulation.

  • 运输(Shipping)

    This product is supplied and shipped with dry ice, please inquire the shipping cost.

  • 存储(Storage)

    Please avoid repeated freeze-thaw cycles.

    This product is stable after storage at:

    1. The product MUST be stored at -70°C or lower upon receipt;
    2. -70°C for 3 months under sterile conditions.
  • 质量管理控制体系(QMS)

    1. 质量管理体系(ISO, GMP)
    2. 质量优势
    3. 质控流程

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数据展示

  • 电泳(SDS-PAGE)

    Prostatic Acid Phosphatase SDS-PAGE

    Biotinylated Human Prostatic Acid Phosphatase (33-382) Protein, His,Avitag on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 90% (With Star Ribbon Pre-stained Protein Marker).

  • SEC-MALS

    Prostatic Acid Phosphatase SEC-MALS

    The purity of Biotinylated Human Prostatic Acid Phosphatase (33-382) Protein, His,Avitag (Cat. No. PRE-H82E9) is more than 95% and the molecular weight of this protein is around 88-118 kDa verified by SEC-MALS.

    Report
  • 活性(Bioactivity)

    Measured by its ability to cleave a substrate, p-Nitrophenyl phosphate (pNPP). The specific activity is >65,000 pmol/min/ug (QC tested).

    Protocol

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背景介绍

Tyrosine phosphatase that acts as a tumor suppressor of prostate cancer through dephosphorylation of ERBB2 and deactivation of MAPK-mediated signaling.In addition to its tyrosine phosphatase activity has ecto-5'-nucleotidase activity in dorsal root ganglion (DRG) neurons. Generates adenosine from AMP which acts as a pain suppressor.

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