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分子别名（Synonym）
Prefusion glycoprotein F0/pre-F protein (RSV)
表达区间及表达系统（Source）
HRSV (A) Pre-fusion glycoprotein F0, His Tag (RSF-V52H7) is expressed from human 293 cells (HEK293). It contains AA Gln 26 - Leu 513 (Accession # P03420).
Predicted N-terminus: Gln 26
蛋白结构（Molecular Characterization）
This protein carries a polyhistidine tag at the C-terminus.
The protein has a calculated MW of 56.1 kDa. The protein migrates as 60-65 kDa when calibrated against Star Ribbon Pre-stained Protein Marker under reducing (R) condition (SDS-PAGE) due to glycosylation.
Within the expressed region, several mutations were introduced to optimize the protein expression and stability.
The protein is designed as a trimer.
内毒素（Endotoxin）
Less than 1.0 EU per μg by the LAL method / rFC method.
纯度（Purity）
>95% as determined by SDS-PAGE.
>90% as determined by SEC-MALS.
制剂（Formulation）
Supplied as 0.2 μm filtered solution in 0.1 M Sodium citrate, 150 mM NaCl, pH5.5 with trehalose as protectant.
Contact us for customized product form or formulation.
运输（Shipping）
This product is supplied and shipped with dry ice, please inquire the shipping cost.
存储（Storage）
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
- The product MUST be stored at -70°C or lower upon receipt;
- -70°C for 3 months under sterile conditions.
质量管理控制体系（QMS）
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数据展示
电泳（SDS-PAGE）
HRSV (A) Pre-fusion glycoprotein F0, His Tag on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 95% (With Star Ribbon Pre-stained Protein Marker).
SEC-MALS
The purity of HRSV (A) Pre-fusion glycoprotein F0, His Tag (Cat. No. RSF-V52H7) is more than 90% and the molecular weight of this protein is around 175-200 kDa verified by SEC-MALS.
Report
活性（Bioactivity）-ELISA
Immobilized HRSV (A) Fusion glycoprotein F0, His Tag (Cat. No. RSF-V52H7) at 2 μg/mL (100 μL/well) can bind Anti-Fusion glycoprotein F0 Antibody, Human IgG1 (D25) with a linear range of 0.2-1 ng/mL (QC tested).
Protocol
Immobilized Monoclonal Anti-HRSV F Antibody, Human IgG1 (AM14) (Cat. No. HRF-S367) at 1 μg/mL (100 μL/well) can bind HRSV (A) Pre-fusion glycoprotein F0, His Tag (Cat. No. RSF-V52H7) with a linear range of 0.2-16 ng/mL (QC tested).
Protocol
Immobilized HRSV (A) Pre-fusion glycoprotein F0, His Tag (MALS verified) (Cat. No. RSF-V52H7) at 2 μg/mL (100 μL/well) can bind Monoclonal Anti-RSV-F0 broadly Antibody, Mouse IgG1 (3B4) with a linear range of 0.098-3.125 ng/mL (Routinely tested).
Protocol
Immobilized HRSV (A) Pre-fusion glycoprotein F0, His Tag (MALS verified) (Cat. No. RSF-V52H7) at 2 μg/mL (100 μL/well) can bind Monoclonal Anti-RSV-F0 broadly Antibody, Mouse IgG1 (4G2) with a linear range of 0.024-0.781 ng/mL (Routinely tested).
Protocol
Immobilized HRSV (A) Fusion glycoprotein F0, His Tag (Cat. No. RSF-V52H7) at 2 μg/mL (100 μL/well) can bind Anti-Fusion glycoprotein F0 Antibody, Mouse IgG2a (101F) with a linear range of 0.2-5 ng/mL (Routinely tested).
Protocol
Immobilized Anti-Fusion glycoprotein F0 Antibody, Mouse IgG2a (101F) at 2 μg/mL (100 μL/well) can bind HRSV (A) Fusion glycoprotein F0, His Tag (Cat. No. RSF-V52H7) with a linear range of 0.1-13 ng/mL (Routinely tested).
Protocol
Immobilized Nirsevimab, Human IgG1 kappa at 1 μg/mL (100 μL/well) can bind HRSV (A) Pre-fusion glycoprotein F0, His Tag (Cat. No. RSF-V52H7) with a linear range of 0.2-8 ng/mL (Routinely tested).
Protocol
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