Biotinylated Mouse Podocalyxin Protein, Fc,Avitag™

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PON-M82F3-25ug
¥3675.00
PON-M82F3-200ug
¥14490.00
合计0件 产品金额¥ 0

产品详情

  • 分子别名(Synonym)

    Gp200, PC, PCLP, PCLP-1, PDX, PODXL1, gp135

  • 表达区间及表达系统(Source)

    Biotinylated Mouse Podocalyxin Protein, Fc,Avitag (PON-M82F3) is expressed from human 293 cells (HEK293). It contains AA Gly 272 - Arg 402 (Accession # Q9R0M4).

    Predicted N-terminus: Gly 272

    Request for sequence
  • 蛋白结构(Molecular Characterization)

    Podocalyxin Structure

    This protein carries a human IgG1 Fc tag at the C-terminus, followed by an Avi tag (Avitag™).

    The protein has a calculated MW of 42.9 kDa. The protein migrates as 45-55 kDa when calibrated against Star Ribbon Pre-stained Protein Marker under reducing (R) condition (SDS-PAGE) due to glycosylation.

  • 标记(Labeling)

    Biotinylation of this product is performed using Avitag™ technology. Briefly, the single lysine residue in the Avitag is enzymatically labeled with biotin.

  • 蛋白标记度(Protein Ratio)

    Passed as determined by the HABA assay / binding ELISA.

  • 纯度(Purity)

    >90% as determined by SDS-PAGE.

  • 制剂(Formulation)

    Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.

    Contact us for customized product form or formulation.

  • 重构方法(Reconstitution)

    Please see Certificate of Analysis for specific instructions.

    For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

  • 存储(Storage)

    For long term storage, the product should be stored at lyophilized state at -20°C or lower.

    Please avoid repeated freeze-thaw cycles.

    This product is stable after storage at:

    1. -20°C to -70°C for 12 months in lyophilized state;
    2. -70°C for 3 months under sterile conditions after reconstitution.
  • 质量管理控制体系(QMS)

    1. 质量管理体系(ISO, GMP)
    2. 质量优势
    3. 质控流程

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  • 电泳(SDS-PAGE)

    Podocalyxin SDS-PAGE

    Biotinylated Mouse Podocalyxin Protein, Fc,Avitag on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 90% (With Star Ribbon Pre-stained Protein Marker).

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背景介绍

Podocalyxin is a heavily glycosylated transmembrane sialoprotein belonging to the CD34 family of cell surface molecules. It is primarily expressed in renal glomerular podocytes, where it plays a critical role in maintaining the integrity of the glomerular filtration barrier through charge-dependent repulsion and modulation of podocyte foot process architecture. The protein’s large extracellular domain, rich in sialic acid residues, mediates interactions with other molecules, influencing cell signaling and migration. Its multifunctional role makes it a potential biomarker and therapeutic target in both renal diseases and cancer research.

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