关注公众号
收藏产品 产品详情
分子别名（Synonym）
Urokinase, PLAU, ATF, UPA, URK, u-PA, BDPLT5, QPD
表达区间及表达系统（Source）
Human PLAU, His Tag (PLU-H5229) is expressed from human 293 cells (HEK293). It contains AA Ser 21 - Leu 431 (Accession # NP_002649.1).
Predicted N-terminus: Ser 21
Request for sequence蛋白结构（Molecular Characterization）
Human PLAU, His Tag is fused with a polyhistidine tag at the C-terminus, and has a calculated MW of 47.2 kDa. The protein migrates as 45-60 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.
Please note that the protein is not activated by protease digestion. In vitro activation is generally recommended for higher activity.
内毒素（Endotoxin）
Less than 1.0 EU per μg by the LAL method / rFC method.
纯度（Purity）
>92% as determined by SDS-PAGE.
制剂（Formulation）
Lyophilized from 0.22 μm filtered solution in 50 mM Sodium Acetate, 100 mM NaCl, pH5.0 with trehalose as protectant.
Contact us for customized product form or formulation.
重构方法（Reconstitution）
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
存储（Storage）
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
- -20°C to -70°C for 12 months in lyophilized state;
- -70°C for 3 months under sterile conditions after reconstitution.
质量管理控制体系（QMS）
推荐产品
数据展示
电泳（SDS-PAGE）
Human PLAU, His Tag on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 92%.
活性（Bioactivity）-ELISA
Immobilized Human PLAU, His Tag (Cat. No. PLU-H5229) at 5 μg/mL (100 μL/well) can bind Biotinylated Human uPAR, His,Avitag (Cat. No. UPR-H82E7) with a linear range of 0.3-5 ng/mL (QC tested).
Protocol
活性（Bioactivity）-SPR
Human uPAR, Fc Tag (Cat. No. UPR-H5254) captured on Protein A Chip can bind Human PLAU / uPA Protein, His Tag (Cat. No. PLU-H5229) with an affinity constant of 10.6 pM as determined in SPR assay (Biacore 8K) (Routinely tested).
Protocol
用户评价 发表评论
药物研发进展 
联系我们 
项目合作 