Biotinylated Human Platelet glycoprotein V Protein, His,Avitag™ (MALS verified)

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PLV-H82E3-25ug
¥3675.00
PLV-H82E3-100ug
¥10290.00
PLV-H82E3-200ug
¥15960.00
合计0件 产品金额¥ 0

产品详情

  • 分子别名(Synonym)

    CD42d, GPV

  • 表达区间及表达系统(Source)

    Biotinylated Human Platelet glycoprotein V Protein, His,Avitag (PLV-H82E3) is expressed from human 293 cells (HEK293). It contains AA Gln 17- Gly 523 (Accession # P40197).

    Predicted N-terminus: Gln 17

    Request for sequence
  • 蛋白结构(Molecular Characterization)

    Platelet glycoprotein V Structure

    This protein carries a polyhistidine tag at the C-terminus, followed by an Avi tag (Avitag™).

    The protein has a calculated MW of 58.6 kDa. The protein migrates as 80-95 kDa when calibrated against Star Ribbon Pre-stained Protein Marker under reducing (R) condition (SDS-PAGE) due to glycosylation.

  • 标记(Labeling)

    Biotinylation of this product is performed using Avitag™ technology. Briefly, the single lysine residue in the Avitag is enzymatically labeled with biotin.

  • 蛋白标记度(Protein Ratio)

    Passed as determined by the HABA assay / binding ELISA.

  • 纯度(Purity)

    >95% as determined by SDS-PAGE.

  • 制剂(Formulation)

    Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.

    Contact us for customized product form or formulation.

  • 重构方法(Reconstitution)

    Please see Certificate of Analysis for specific instructions.

    For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

  • 存储(Storage)

    For long term storage, the product should be stored at lyophilized state at -20°C or lower.

    Please avoid repeated freeze-thaw cycles.

    This product is stable after storage at:

    1. -20°C to -70°C for 12 months in lyophilized state;
    2. -70°C for 3 months under sterile conditions after reconstitution.
  • 质量管理控制体系(QMS)

    1. 质量管理体系(ISO, GMP)
    2. 质量优势
    3. 质控流程

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数据展示

  • 电泳(SDS-PAGE)

    Platelet glycoprotein V SDS-PAGE

    Biotinylated Human Platelet glycoprotein V Protein, His,Avitag on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 95% (With Star Ribbon Pre-stained Protein Marker).

  • SEC-MALS

    Platelet glycoprotein V SEC-MALS

    The purity of Biotinylated Human Platelet glycoprotein V Protein, His,Avitag (Cat. No. PLV-H82E3) is more than 85% and the molecular weight of this protein is around 65-85 kDa verified by SEC-MALS.

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背景介绍

Human platelet glycoprotein V (GP5) is a part of the Ib-V-IX system of surface glycoproteins that constitute the receptor for von Willebrand factor (VWF; MIM 613160) and mediate the adhesion of platelets to injured vascular surfaces in the arterial circulation, a critical initiating event in hemostasis. The main portion of the receptor is a heterodimer composed of 2 polypeptide chains, an alpha chain (GP1BA; MIM 606672) and a beta chain (GP1BB; MIM 138720), that are linked by disulfide bonds. The complete receptor complex includes noncovalent association of the alpha and beta subunits with platelet glycoprotein IX (GP9; MIM 173515) and GP5. Mutations in GP1BA, GP1BB, and GP9 have been shown to cause Bernard-Soulier syndrome (MIM 231200), a bleeding disorder (review by Lopez et al., 1998 [PubMed 9616133]).[supplied by OMIM, Nov 2010]

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