关注公众号
收藏产品 产品详情
产品描述（Product Details）
Phi29 DNA Polymerase is a DNA polymerase cloned from the Bacillus subtilis phage Phi29, expressed and purified in Escherichia coli. This enzyme exhibits remarkable strand displacement activity and high processivity, enabling it to synthesize DNA fragments of up to 70 kb in length. It also demonstrates strong template binding capability and possesses robust 3′→5′ exonuclease proofreading activity.
应用说明（Application）
Whole Genome Amplification (WGA).
Rolling Circle Amplification (RCA).
Long DNA Fragment Synthesis.
单位定义（Unit Definition）
One unit is defined as the amount of enzyme that will incorporate 0.5 pmol of dNTP into acid insoluble material in 10 minutes at 30°C.
纯度（Purity）
>95% as determined by SDS-PAGE.
酶活（Enzyme Activity）
>10 U/μL
制剂（Formulation）
Supplied as 0.2 μm filtered solution in 10 mM Tris, pH7.4 with glycerol as protectant.
Contact us for customized product form or formulation.
运输（Shipping）
This product is supplied and shipped with dry ice, please inquire the shipping cost.
存储（Storage）
This product is stable after storage at:
- The product MUST be stored at -20°C or lower upon receipt;
- -20°C for 3 months under sterile conditions.
- *该试剂盒配套10× Reaction Buffer中含有DTT，DTT对酶活影响较大，当使用长期贮存或反复冻融的10× Reaction Buffer时，为了保证最大活性，应补加40 mM DTT。
推荐产品
数据展示
电泳（SDS-PAGE）
The gel was stained with Coomassie Blue. The purity of the protein is greater than 95% (With Star Ribbon Pre-stained Protein Marker).
活性（Bioactivity）
One unit is defined as the amount of enzyme that will incorporate 0.5 pmol of dNTP into acid insoluble material in 10 minutes at 30°C (QC tested).
用户评价 发表评论
联系我们 
项目合作 