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分子别名（Synonym）
PDL2,PD-L2,Butyrophilin B7-DC,CD273,PDCD1 ligand 2,PDCD1L2,PDCD1LG2
表达区间及表达系统（Source）
Human PD-L2, Mouse IgG1 Fc Tag (PD2-H52A5) is expressed from human 293 cells (HEK293). It contains AA Leu 20 - Pro 219 (Accession # AAI13679).
Predicted N-terminus: Leu 20
Request for sequence蛋白结构（Molecular Characterization）
This protein carries a mouse IgG1 Fc tag at the C-terminus.
The protein has a calculated MW of 48.9 kDa. The protein migrates as 60-70 kDa when calibrated against Star Ribbon Pre-stained Protein Marker under reducing (R) condition (SDS-PAGE) due to glycosylation.
内毒素（Endotoxin）
Less than 1.0 EU per μg by the LAL method / rFC method.
纯度（Purity）
>95% as determined by SDS-PAGE.
制剂（Formulation）
Lyophilized from 0.22 μm filtered solution in Tris with Glycine, Arginine and NaCl, pH7.5 with trehalose as protectant.
Contact us for customized product form or formulation.
重构方法（Reconstitution）
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
存储（Storage）
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
- -20°C to -70°C for 12 months in lyophilized state;
- -70°C for 3 months under sterile conditions after reconstitution.
质量管理控制体系（QMS）
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数据展示
电泳（SDS-PAGE）
Human PD-L2, Mouse IgG1 Fc Tag on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 95% (With Star Ribbon Pre-stained Protein Marker).
活性（Bioactivity）-ELISA
Immobilized Human PD-1, His Tag (Cat. No. PD1-H5221) at 2 μg/mL (100 μL/well) can bind Human PD-L2, Mouse IgG1 Fc Tag (Cat. No. PD2-H52A5) with a linear range of 10-156 ng/mL (QC tested).
Protocol
Serial dilutions of nivolumab were added into Human PD-L2, Mouse IgG1 Fc Tag (Cat. No. PD2-H52A5): Biotinylated Human PD-1, Fc,Avitag (Cat. No. PD1-H82F1) binding reactions. The half maximal inhibitory concentration (IC50) is 0.2823 μg/mL (Routinely tested).
Protocol
活性（Bioactivity）-FACS
Flow Cytometry assay shows that Human PD-L2, Mouse IgG1 Fc Tag (Cat. No. PD2-H52A5) can bind to 293 cell overexpressing human PD-1. The concentration of PD-L2 used is 0.1 μg/mL (Routinely tested).
Protocol
FACS analysis shows that the binding of Human PD-L2, Mouse IgG1 Fc Tag (Cat. No. PD2-H52A5) to 293 overexpressing PD-1 was inhibited by increasing concentration of neutralizing Anti-PD-1 antibody. The concentration of PD-L2 used is 0.1 μg/mL. The IC50 is 1.3 μg/mL (Routinely tested).
Protocol
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