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Rat PD-1 / PDCD1 Protein, Fc Tag (MALS verified)

COADS MS
DS
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PD1-R5253-100ug
¥3350.00
PD1-R5253-1mg
¥20790.00
合计0件 产品金额¥ 0
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产品详情

  • 分子别名（Synonym）

    PDCD1,PD1,CD279,SLEB2

  • 表达区间及表达系统（Source）

    Rat PD-1, Fc Tag (PD1-R5253) is expressed from human 293 cells (HEK293). It contains AA Leu 25 - Gln 167 (Accession # NP_001100397.1).

    Predicted N-terminus: Leu 25

    Request for sequence

  • 蛋白结构（Molecular Characterization）

    PD-1 Structure

    This protein carries a human IgG1 Fc tag at the C-terminus.

    The protein has a calculated MW of 42.9 kDa. The protein migrates as 55-66 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

  • 内毒素（Endotoxin）

    Less than 1.0 EU per μg by the LAL method / rFC method.

  • 纯度（Purity）

    >95% as determined by SDS-PAGE.

  • 制剂（Formulation）

    Lyophilized from 0.22 μm filtered solution in Tris with Glycine, Arginine and NaCl, pH7.5 with trehalose as protectant.

    Contact us for customized product form or formulation.

  • 重构方法（Reconstitution）

    Please see Certificate of Analysis for specific instructions.

    For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

  • 存储（Storage）

    For long term storage, the product should be stored at lyophilized state at -20°C or lower.

    Please avoid repeated freeze-thaw cycles.

    This product is stable after storage at:

    1. -20°C to -70°C for 12 months in lyophilized state;
    2. -70°C for 3 months under sterile conditions after reconstitution.

  • 质量管理控制体系（QMS）

    1. 质量管理体系（ISO, GMP）
    2. 质量优势
    3. 质控流程

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数据展示

  • 电泳（SDS-PAGE）

    PD-1 SDS-PAGE

    Rat PD-1, Fc Tag on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 95%.

  • SEC-MALS

    PD-1 SEC-MALS

    The purity of Rat PD-1, Fc Tag (Cat. No. PD1-R5253) is more than 85% and the molecular weight of this protein is around 105-125 kDa verified by SEC-MALS.

    Report

  • 活性（Bioactivity）-ELISA

    PD-1 ELISA

    Immobilized Mouse PD-L2, Mouse IgG2a Fc Tag, low endotoxin at 2 μg/mL (100 μL/well) can bind Rat PD-1, Fc Tag (Cat. No. PD1-R5253) with a linear range of 5-20 ng/mL (QC tested).

    Protocol

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背景介绍

Programmed cell death protein 1 (PD-1) is also known as CD279 and PDCD1, is a type I membrane protein and is a member of the extended CD28/CTLA-4 family of T cell regulators. PDCD1 is expressed on the surface of activated T cells, B cells, macrophages, myeloid cells and a subset of thymocytes. PD-1 has two ligands, PD-L1 and PD-L2, which are members of the B7 family. PD-L1 is expressed on almost all murine tumor cell lines, including PA1 myeloma, P815 mastocytoma, and B16 melanoma upon treatment with IFN-γ. PD-L2 expression is more restricted and is expressed mainly by DCs and a few tumor lines. PD1 inhibits the T-cell proliferation and production of related cytokines including IL-1, IL-4, IL-10 and IFN-γ by suppressing the activation and transduction of PI3K/AKT pathway. In addition, coligation of PD1 inhibits BCR-mediating signal by dephosphorylating key signal transducer. In vitro, treatment of anti-CD3 stimulated T cells with PD-L1-Ig results in reduced T cell proliferation and IFN-γ secretion. Monoclonal antibodies targeting PD-1 that boost the immune system are being developed for the treatment of cancer.

前沿进展

 
药物研发进展
  • 英文全称：

    Programmed cell death protein 1

  • 中文全称：

    细胞程序性死亡-1

  • 种类：

  • 上市药物数量：

    20 详情

  • 临床药物数量：

    167 详情

  • 最高研发阶段：

    临床三期

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