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分子别名（Synonym）
PDCD1, PD1, CD279, SLEB2
表达区间及表达系统（Source）
Mouse PD-1, Fc Tag (PD1-M5259) is expressed from human 293 cells (HEK293). It contains AA Leu 25 - Gln 167 (Accession # NP_032824).
Predicted N-terminus: Leu 25
Request for sequence蛋白结构（Molecular Characterization）
Other Tags and Version Biotin & Other Labeled Version
This protein carries a human IgG1 Fc tag at the C-terminus.
The protein has a calculated MW of 42.8 kDa. The protein migrates as 55-66 kDa when calibrated against Star Ribbon Pre-stained Protein Marker under reducing (R) condition (SDS-PAGE) due to glycosylation.
内毒素（Endotoxin）
Less than 0.1 EU per μg by the LAL method / rFC method.
纯度（Purity）
>95% as determined by SDS-PAGE.
>90% as determined by SEC-MALS.
制剂（Formulation）
Lyophilized from 0.22 μm filtered solution in Tris with Glycine, Arginine and NaCl, pH7.5 with trehalose as protectant.
Contact us for customized product form or formulation.
重构方法（Reconstitution）
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
存储（Storage）
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
- -20°C to -70°C for 12 months in lyophilized state;
- -70°C for 3 months under sterile conditions after reconstitution.
质量管理控制体系（QMS）
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数据展示
电泳（SDS-PAGE）
Mouse PD-1, Fc Tag on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 95% (With Star Ribbon Pre-stained Protein Marker).
SEC-MALS
The purity of Mouse PD-1, Fc Tag (Cat. No. PD1-M5259) is more than 90% and the molecular weight of this protein is around 105-130 kDa verified by SEC-MALS.
Report
活性（Bioactivity）-ELISA
Immobilized Mouse PD-L1, Mouse IgG2a Fc Tag, low endotoxin (Cat. No. PD1-M52A2) at 2 μg/mL (100 μL/well) can bind Mouse PD-1, Fc Tag (Cat. No. PD1-M5259) with a linear range of 0.039-0.625 μg/mL (QC tested).
Protocol
活性（Bioactivity）-SPR
Captured Mouse PD-1, Fc Tag (Cat. No. PD1-M5259) on Protein A Chip can bind Mouse PD-L1, His Tag (Cat. No. PD1-M5220) with an affinity constant of 2.16 μM as determined in SPR assay (Biacore T200) (Routinely tested).
Protocol
Captured Mouse PD-L1, His Tag (Cat. No. PD1-M5220) on CM5 Chip via Anti-His antibody can bind Mouse PD-1, Fc Tag (Cat. No. PD1-M5259) with an affinity constant of 1.37 μM as determined in SPR assay (Biacore T200) (Routinely tested).
Protocol
活性（Bioactivity）-BLI
Loaded Mouse PD-1, Fc Tag (Cat. No. PD1-M5259) on Protein A Biosensor, can bind Mouse PD-L1, His Tag (Cat. No. PD1-M5220) with an affinity constant of 1.5 μM as determined in BLI assay (ForteBio Octet Red96e) (Routinely tested).
Protocol
Loaded Mouse PD-1, Fc Tag (Cat. No. PD1-M5259) on Protein A Biosensor, can bind Mouse PD-L2, His Tag (Cat. No. PD2-M52E3) with an affinity constant of 0.13 μM as determined in BLI assay (ForteBio Octet Red96e) (Routinely tested).
Protocol
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