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分子别名（Synonym）
Papain-like Protease, Replicase polyprotein 1a, ORF1a polyprotein, pp1a, 1a, PL-PRO, Plpro
表达区间及表达系统（Source）
SARS-CoV-2 Papain-like Protease, His Tag (PAE-C5148) is expressed from E. coli cells. It contains AA Glu 746 - Lys 1060 (Accession # YP_009725299.1).
Predicted N-terminus: Met
Request for sequence蛋白结构（Molecular Characterization）
This protein carries a polyhistidine tag at the N-terminus.
The protein has a calculated MW of 37.6 kDa. The protein migrates as 35-37 kDa under reducing (R) condition (SDS-PAGE).
纯度（Purity）
>95% as determined by SDS-PAGE.
制剂（Formulation）
Supplied as 0.2 μm filtered solution in 50 mM HEPES, 300 mM NaCl, pH7.5, 11% Trehalose with glycerol as protectant.
Contact us for customized product form or formulation.
运输（Shipping）
This product is supplied and shipped with dry ice, please inquire the shipping cost.
存储（Storage）
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
- The product MUST be stored at -70°C or lower upon receipt;
- -70°C for 3 months under sterile conditions.
质量管理控制体系（QMS）
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数据展示
电泳（SDS-PAGE）
SARS-CoV-2 Papain-like Protease, His Tag on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 95%.
活性（Bioactivity）
Measured by its ability to convert the substrate Dabcyl–FRLKGGAPIKGV–Edans.The specific activity is >2500 pmol/min/mg (QC tested).
Protocol
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