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分子别名（Synonym）
TNFRSF4, OX40, CD134, OX40L receptor, ACT35, TXGP1L
表达区间及表达系统（Source）
Mouse OX40, Fc Tag (OX0-M5259) is expressed from human 293 cells (HEK293). It contains AA Val 20 - Pro 211 (Accession # NP_035789).
Predicted N-terminus: Val 20
Request for sequence蛋白结构（Molecular Characterization）
Other Tags and Version Biotin & Other Labeled Version
This protein carries a human IgG1 Fc tag at the C-terminus.
The protein has a calculated MW of 47.9 kDa. The protein migrates as 55-70 kDa when calibrated against Star Ribbon Pre-stained Protein Marker under reducing (R) condition (SDS-PAGE) due to glycosylation.
内毒素（Endotoxin）
Less than 1.0 EU per μg by the LAL method / rFC method.
纯度（Purity）
>90% as determined by SDS-PAGE.
>95% as determined by SEC-MALS.
制剂（Formulation）
Lyophilized from 0.22 μm filtered solution in Tris with Glycine, Arginine and NaCl, pH7.5 with trehalose as protectant.
Contact us for customized product form or formulation.
重构方法（Reconstitution）
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
存储（Storage）
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
- -20°C to -70°C for 12 months in lyophilized state;
- -70°C for 3 months under sterile conditions after reconstitution.
质量管理控制体系（QMS）
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数据展示
电泳（SDS-PAGE）
Mouse OX40, Fc Tag on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 90% (With Star Ribbon Pre-stained Protein Marker).
SEC-MALS
The purity of Mouse OX40, Fc Tag (Cat. No. OX0-M5259) is more than 95% and the molecular weight of this protein is around 110-145 kDa verified by SEC-MALS.
Report
活性（Bioactivity）-ELISA
Immobilized Mouse OX40 Ligand, His Tag (Cat. No. TN4-M5241) at 0.5 μg/mL (100 μL/well) can bind Mouse OX40, Fc Tag (Cat. No. OX0-M5259) with a linear range of 0.2-16 ng/mL (QC tested).
Protocol
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