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分子别名（Synonym）
Nucleocapsid protein/NP (Influenza Virus)
表达区间及表达系统（Source）
Influenza A virus (Wisconsin/67/2022) NP (H1N1) Protein, His Tag (NP1-V52H3) is expressed from human 293 cells (HEK293). It contains AA Met 1 - Asn 498 (Accession # EPI2224781, GISAID).
Request for sequence蛋白结构（Molecular Characterization）
This protein carries a polyhistidine tag at the C-terminus.
The protein has a calculated MW of 58.1 kDa. The protein migrates as 60-65 kDa when calibrated against Star Ribbon Pre-stained Protein Marker under reducing (R) condition (SDS-PAGE) due to glycosylation.
内毒素（Endotoxin）
Less than 1.0 EU per μg by the LAL method / rFC method.
纯度（Purity）
>90% as determined by SDS-PAGE.
制剂（Formulation）
Lyophilized from 0.22 μm filtered solution in 0.2 M Arginine, PBS, pH7.4 with trehalose as protectant.
Contact us for customized product form or formulation.
重构方法（Reconstitution）
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
存储（Storage）
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
- -20°C to -70°C for 12 months in lyophilized state;
- -70°C for 3 months under sterile conditions after reconstitution.
质量管理控制体系（QMS）
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数据展示
电泳（SDS-PAGE）
Influenza A virus (Wisconsin/67/2022) NP (H1N1) Protein, His Tag on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 90% (With Star Ribbon Pre-stained Protein Marker).
活性（Bioactivity）-ELISA
Immobilized Influenza A virus (Wisconsin/67/2022) NP (H1N1) Protein, His Tag (Cat. No. NP1-V52H3) at 1 μg/mL (100 μL/well) can bind Influenza A Nucleoprotein Antibody, Mouse MAb with a linear range of 0.05-1.56 ng/mL (QC tested).
Protocol
Immobilized Influenza A Nucleoprotein Antibody, Mouse MAb at 1 μg/mL (100 μL/well) can bind Influenza A virus (Wisconsin/67/2022) NP (H1N1) Protein, His Tag (Cat. No. NP1-V52H3) with a linear range of 1-63 ng/mL (Routinely tested).
Protocol
活性（Bioactivity）-SPR
Influenza A Nucleoprotein Antibody, Mouse MAb immobilized on CM4 Chip can bind Influenza A virus (Wisconsin/67/2022) NP (H1N1) Protein, His Tag (Cat. No. NP1-V52H3) with an affinity constant of 3.32 nM as determined in a SPR assay (Biacore 8K) (Routinely tested).
Protocol
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