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分子别名（Synonym）
HTX5
表达区间及表达系统（Source）
Human NODAL Protein, Tag Free (NOL-H5133) is expressed from E. coli cells. It contains AA His 238 - Leu 347 (Accession # Q96S42).
Predicted N-terminus: Met
Request for sequence蛋白结构（Molecular Characterization）
Other Tags and Version Biotin & Other Labeled Version
This protein carries no "tag".
The protein has a calculated MW of 12.9 kDa. The protein migrates as 14-15 kDa when calibrated against Star Ribbon Pre-stained Protein Marker under reducing (R) condition (SDS-PAGE).
内毒素（Endotoxin）
Less than 1.0 EU per μg by the LAL method / rFC method.
纯度（Purity）
>90% as determined by SDS-PAGE.
制剂（Formulation）
Lyophilized from 0.22 μm filtered solution in 10 mM Sodium citrate, pH3.0 with trehalose as protectant.
Contact us for customized product form or formulation.
重构方法（Reconstitution）
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
存储（Storage）
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
- -20°C to -70°C for 12 months in lyophilized state;
- -70°C for 3 months under sterile conditions after reconstitution.
质量管理控制体系（QMS）
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数据展示
电泳（SDS-PAGE）
Human NODAL Protein, Tag Free on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 90% (With Star Ribbon Pre-stained Protein Marker).
活性（Bioactivity）-ELISA
Immobilized Human NODAL Protein, Tag Free (Cat. No. NOL-H5133) at 10 μg/mL (100 μL/well) can bind TDGF1 Protein, Human (HEK293, Fc) with a linear range of 0.005-0.313 μg/mL (QC tested).
Protocol
Immobilized Human NODAL Protein, Tag Free (Cat. No. NOL-H5133) at 5 μg/mL (100 μL/well) can bind Human ACVR2A, Fc Tag (Cat. No. ACA-H5269) with a linear range of 0.01-0.313 μg/mL (Routinely tested).
Protocol
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