Human NK2R Full Length Protein, Flag,His Tag (Detergent)

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NKR-H52D3-20ug
¥7350.00
NKR-H52D3-100ug
¥27300.00
NKR-H52D3-500ug
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产品详情

  • 分子别名(Synonym)

    NK2R, NKNAR, SKR, TAC2R

  • 表达区间及表达系统(Source)

    Human NK2R Full Length Protein, Flag,His Tag (NKR-H52D3) is expressed from human 293 cells (HEK293). It contains AA Gly 2 - Ile 398 (Accession # P21452).

    Predicted N-terminus: Asp

    Request for sequence
  • 蛋白结构(Molecular Characterization)

    NK2R Structure

    This protein carries a flag tag at the N-terminus and a polyhistidine tag at the C-terminus.

    The protein has a calculated MW of 47.9 kDa. The protein migrates as 53-55 kDa and 65-70 kDa when calibrated against Star Ribbon Pre-stained Protein Marker under reducing (R) condition (SDS-PAGE) due to glycosylation.

  • 纯度(Purity)

    >90% as determined by SDS-PAGE.

  • 制剂(Formulation)

    This product is not suitable for cell based experiments due to cytotoxicity of detergent.
    Detergent buffer is INDISPENSABLE to keep membrane protein soluble and active, under no circumstances should you remove detergent.
    Detergent buffer is sold separately and not included in protein, and please contact us if you need the buffer.
    If glycerol is not compatible with your application, remove glycerol just before the immediate experiment, and NEVER store glycerol-free protein solution.

    Supplied as 0.2 μm filtered solution in 50 mM HEPES, 150 mM NaCl, Buffer B, CHS, pH7.5 with glycerol as protectant.

    Contact us for customized product form or formulation.

  • 运输(Shipping)

    This product is supplied and shipped with dry ice, please inquire the shipping cost.

  • 存储(Storage)

    Please avoid repeated freeze-thaw cycles.

    This product is stable after storage at:

    1. The product MUST be stored at -70°C or lower upon receipt;
    2. -70°C for 3 months under sterile conditions.
  • 质量管理控制体系(QMS)

    1. 质量管理体系(ISO, GMP)
    2. 质量优势
    3. 质控流程
  • *The detergent Buffer B (Cat. No. LG-13) is sold separately and not included in protein, you can follow the link for product information.

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数据展示

  • 电泳(SDS-PAGE)

    NK2R SDS-PAGE

    Human NK2R Full Length Protein, Flag,His Tag on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 90% (With Star Ribbon Pre-stained Protein Marker).

  • 活性(Bioactivity)-ELISA

     NK2R ELISA

    Immobilized Human NK2R Full Length Protein, Flag,His Tag (Cat. No. NKR-H52D3) at 1 μg/mL (100 μL/well) can bind Anti-NK2R Antibody, Rabbit IgG with a linear range of 0.02-1.25 μg/mL (QC tested).

    Protocol

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背景介绍

NK2R is a G protein-coupled receptor with high affinity for the tachykinin Substance K (Neurokinin A). Upon activation, it triggers a phosphatidylinositol-calcium signaling pathway, leading to intracellular calcium release. The receptor's binding affinity follows the order: Substance K > Neuromedin K > Substance P. NK2R also regulates energy metabolism and has shown significant potential in appetite control. Due to its diverse functions, it is considered a promising therapeutic target for conditions such as asthma, irritable bowel syndrome, obesity, and type 2 diabetes.

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