Human NFL Protein, His Tag (HPLC verified)

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货号-规格
价格
Qty.
NFL-H51H4-100ug
¥3800.00
NFL-H51H4-500ug (250ug X 2)
¥11500.00
合计0件 产品金额¥ 0

产品详情

  • 分子别名(Synonym)

    Neurofilament light polypeptide

  • 表达区间及表达系统(Source)

    Human NFL Protein, His Tag (NFL-H51H4) is expressed from E. coli cells. It contains AA Ser 2 - Asp 543 (Accession # P07196-1).

    Predicted N-terminus: Met

    Request for sequence
  • 蛋白结构(Molecular Characterization)

    NFL Structure

    This protein carries a polyhistidine tag at the C-terminus.

    The protein has a calculated MW of 63.4 kDa. The protein migrates as 65-80 kDa when calibrated against Star Ribbon Pre-stained Protein Marker under reducing (R) condition (SDS-PAGE).

  • 纯度(Purity)

    >95% as determined by SDS-PAGE.

    >90% as determined by SEC-HPLC.

  • 制剂(Formulation)

    Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.

    Contact us for customized product form or formulation.

  • 重构方法(Reconstitution)

    Please see Certificate of Analysis for specific instructions.

    For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

  • 存储(Storage)

    For long term storage, the product should be stored at lyophilized state at -20°C or lower.

    Please avoid repeated freeze-thaw cycles.

    This product is stable after storage at:

    1. -20°C to -70°C for 12 months in lyophilized state;
    2. -70°C for 3 months under sterile conditions after reconstitution.
  • 质量管理控制体系(QMS)

    1. 质量管理体系(ISO, GMP)
    2. 质量优势
    3. 质控流程

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数据展示

  • 电泳(SDS-PAGE)

    NFL SDS-PAGE

    Human NFL Protein, His Tag on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 95% (With Star Ribbon Pre-stained Protein Marker).

  • SEC-HPLC

    NFL SEC-HPLC

    The purity of Human NFL Protein, His Tag (Cat. No. NFL-H51H4) was greater than 90% as determined by SEC-HPLC.

  • 活性(Bioactivity)-ELISA

     NFL ELISA

    Immobilized Human NFL Protein, His Tag (Cat. No. NFL-H51H4) at 1 μg/mL (100 μL/well) can bind NFL Monoclonal Antibody with a linear range of 0.06-4 ng/mL (QC tested).

    Protocol

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背景介绍

Neurofilaments (NF) are classed as type IV intermediate filaments found in the cytoplasm of neurons. Together with microtubules and microfilaments, they form the neuronal cytoskeleton. Neurofilaments usually contain three intermediate filament proteins: NFL, NFM, and NFH which are involved in the maintenance of neuronal caliber. Neurofilament heavy (NFH) can server as biomarkers for several diseases by indicating axonal injury.

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