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应用说明（Application）
- Cell based assay (FACS or functional assay)
- Affinity testing (SPR / BLI)
- Screening (ELISA)
- Immunization
分子别名（Synonym）
MSP1D1
表达区间及表达系统（Source）
Nanodisc Human MSP1D1 Protein, His Tag (APO-H51H3) is expressed from E. coli cells. It contains AA Gly 1 - Gln 211.
Predicted N-terminus: Met
蛋白结构（Molecular Characterization）
This protein carries a polyhistidine tag at the N-terminus. The protein has a calculated MW of 24.7 kDa. The protein migrates as 23 kDa and 25 kDa when calibrated against Star Ribbon Pre-stained Protein Marker under reducing (R) condition (SDS-PAGE).
This protein is used together with nanodisc protein as isotype control.
Structure
Nanodisc membrane protein is wrapped by a synthetic phospholipid bilayer membrane structure, composed of membrane scaffold proteins (MSPs) and phospholipids.
Synthesis Process
Nanodisc reconstitution is a self-assembly process that starts from purified, detergent-solubilized membrane proteins and reconstitutes them into synthetic phospholipid bilayers stabilized by MSP1D1 membrane scaffold protein. This creates a stable, detergent-free environment that preserves native conformation and biological activity, with excellent homogeneity and full compatibility with cell-based assays such as FACS and binding assays in antibody discovery and screening (ELISA),etc.
纯度（Purity）
>90% as determined by SDS-PAGE.
制剂（Formulation）
Supplied as 0.2 μm filtered solution in 20 mM HEPES, 150 mM NaCl, pH7.5 with trehalose as protectant.
Contact us for customized product form or formulation.
运输（Shipping）
This product is supplied and shipped with dry ice, please inquire the shipping cost.
存储（Storage）
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
- The product MUST be stored at -70°C or lower upon receipt;
- -70°C for 12 months under sterile conditions.
质量管理控制体系（QMS）
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数据展示
电泳（SDS-PAGE）
Human MSP1D1 Protein, His Tag on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 90% (With Star Ribbon Pre-stained Protein Marker).
活性（Bioactivity）-FACS
2e5 of CD20-CAR-293 cells transfected with anti-CD20-scFv were stained with 100 μL of 10 μg/mL of Human MSP1D1 Protein, His Tag (Cat. No. APO-H51H3), washed and then followed by PE anti-His antibody and analyzed with FACS. PE anti-His antibody was used as negative control (QC tested).
Protocol
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