Human MRGPRX2 Full Length Protein (VLP)

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MR2-H52P3-20ug
¥5250.00
MR2-H52P3-100ug
¥10500.00
MR2-H52P3-500ug (250ug X 2)
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合计0件 产品金额¥ 0

产品详情

  • 分子别名(Synonym)

    MRGPRX2

  • 表达区间及表达系统(Source)

    Human MRGPRX2 Full Length Protein (VLP) (MR2-H52P3) is expressed from human 293 cells (HEK293). It contains AA Asp 2 - Val 330 (Accession # Q96LB1).

    Predicted N-terminus: Asp

  • 蛋白结构(Molecular Characterization)

    Virus-like particles(VLPs) are formed by self-assembly of capsid proteins from viruses. Membrane Proteins can be constituted in-situ with VLPs produced from HEK293 cell cultures. These VLPs concentrate conformationally intact membrane proteins directly on the cell surface and produce soluble, high-concentration proteins perfect for immunization and antibody screening.

    The VLPs provide the display of properly folded membrane proteins in their native cellular membrane in a compact size of 100~300 nm diameter (similar to the size of most viruses) making it optimal targets for dendritic cells in vivo and surface attachment for phage display.

  • 内毒素(Endotoxin)

    Less than 1.0 EU per μg by the LAL method / rFC method.

  • 制剂(Formulation)

    The VLPs are highly immunogenic, so the immunization strategy should be optimized (antigen dose, regimen and adjuvant).

    Supplied as 0.2 μm filtered solution in PBS, Arginine, pH7.4 with trehalose as protectant.

    Contact us for customized product form or formulation.

  • 运输(Shipping)

    This product is supplied and shipped with dry ice, please inquire the shipping cost.

  • 存储(Storage)

    Please avoid repeated freeze-thaw cycles.

    This product is stable after storage at:

    1. The product MUST be stored at -70°C or lower upon receipt;
    2. -70°C for 12 months under sterile conditions.
  • 质量管理控制体系(QMS)

    1. 质量管理体系(ISO, GMP)
    2. 质量优势
    3. 质控流程
  • *The isotype control of empty/mock VLP (Cat. No. VLP-N5213) is sold separately and not included in protein, you can follow this link for product information.

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  • 活性(Bioactivity)-ELISA

     MRGPRX2 ELISA

    Immobilized Human MRGPRX2 Full Length Protein (VLP) (Cat. No. MR2-H52P3) at 1 μg/mL (100 μL/well) can bind Anti-MRGPRX2 Antibody, Mouse IgG2b with a linear range of 0.02-1 ng/mL (QC tested).

    Protocol
  • 均一性(Identity)-DLS

     MRGPRX2 DLS

    The mean peak Radius of VLP is 60-80 nm with more than 95% intensity as determined by dynamic light scattering (DLS).

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背景介绍

The protein belongs to the family of G-protein coupled receptors, that are preferentially activated by adenosine and uridine nucleotides. It is moderately expressed in undifferentiated HL60 cells, and is located on both chromosomes X and Y.

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