Human MRGPRX2 Full Length Protein, Flag Tag (Nanodisc-pro)

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MR2-H52D3-20ug
¥6240.00
MR2-H52D3-100ug
¥12480.00
MR2-H52D3-500ug
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  • 分子别名(Synonym)

    MRGPRX2

  • 表达区间及表达系统(Source)

    Human MRGPRX2 Full Length Protein, Flag Tag (MR2-H52D3) is expressed from human 293 cells (HEK293). It contains AA Asp 2 - Val 330 (Accession # Q96LB1).

    Predicted N-terminus: Asp

    Request for sequence
  • 蛋白结构(Molecular Characterization)

    MRGPRX2 Structure

    This protein carries a flag tag at the N-terminus. The protein has a calculated MW of 53.3 kDa.

    Nanodiscs are a new class of model membranes that are being used to solubilize and study a range of integral membrane proteins and membrane-associated proteins. The Nanodisc bilayer is bounded by an amphipathic scaffold coat that confers enhanced stability and a narrow particle size distribution.

    The nanodisc-pro is directly extracted from cell membrane in a water-soluble form using an amphipathic scaffold followed by affinity chromatography, and thus, it conserves a patch of native membrane around them.

  • 纯度(Purity)

    >90% as determined by SDS-PAGE.

  • 制剂(Formulation)

    Supplied as 0.2 μm filtered solution in 20 mM HEPES, 150 mM NaCl, pH7.5 with glycerol as protectant.

    Contact us for customized product form or formulation.

  • 运输(Shipping)

    This product is supplied and shipped with dry ice, please inquire the shipping cost.

  • 存储(Storage)

    Please avoid repeated freeze-thaw cycles.

    This product is stable after storage at:

    1. The product MUST be stored at -70°C or lower upon receipt;
    2. -70°C for 3 months under sterile conditions.
  • 质量管理控制体系(QMS)

    1. 质量管理体系(ISO, GMP)
    2. 质量优势
    3. 质控流程
  • *The isotype control of empty/mock nanodisc (Cat. No. APO-H5143) is sold separately and not included in protein, you can follow this link for product information.

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  • 活性(Bioactivity)-ELISA

     MRGPRX2 ELISA

    Immobilized Human MRGPRX2 Full Length Protein, Flag Tag (Cat. No. MR2-H52D3) at 1 μg/mL (100 μL/well) can bind Anti-MRGPRX2 Antibody, Mouse IgG2b with a linear range of 0.02-1 ng/mL (QC tested).

    Protocol

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背景介绍

The protein belongs to the family of G-protein coupled receptors, that are preferentially activated by adenosine and uridine nucleotides. It is moderately expressed in undifferentiated HL60 cells, and is located on both chromosomes X and Y.

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