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分子别名（Synonym）
MSLN,Mesothelin,MPF
表达区间及表达系统（Source）
Rat Mesothelin (298-600), His Tag (MSN-R52H4) is expressed from human 293 cells (HEK293). It contains AA Asp 298 - Ser 600 (Accession # XP_032768340.1).
Predicted N-terminus: Asp 298
Request for sequence蛋白结构（Molecular Characterization）
This protein carries a polyhistidine tag at the C-terminus.
The protein has a calculated MW of 35.9 kDa. The protein migrates as 43-48 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.
内毒素（Endotoxin）
Less than 1.0 EU per μg by the LAL method / rFC method.
纯度（Purity）
>95% as determined by SDS-PAGE.
>90% as determined by SEC-MALS.
制剂（Formulation）
Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.
Contact us for customized product form or formulation.
重构方法（Reconstitution）
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
存储（Storage）
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
- -20°C to -70°C for 12 months in lyophilized state;
- -70°C for 3 months under sterile conditions after reconstitution.
质量管理控制体系（QMS）
数据展示
电泳（SDS-PAGE）
Rat Mesothelin (298-600), His Tag on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 95%.
SEC-MALS
The purity of Rat Mesothelin (298-600), His Tag (Cat. No. MSN-R52H4) is more than 90% and the molecular weight of this protein is around 34-52 kDa verified by SEC-MALS.
Report
活性（Bioactivity）-ELISA
Immobilized Rat Mesothelin (298-600), His Tag (Cat. No. MSN-R52H4) at 2 μg/mL (100 μL/well) can bind Monoclonal Anti-Human MSLN Antibody, Human IgG1 with a linear range of 8-125 ng/mL (QC tested).
Protocol
Immobilized Rat Mesothelin (298-600), His Tag (Cat. No. MSN-R52H4) at 2 μg/mL (100 μL/well) can bind Biotinylated Human CA125, Fc,Avitag (Cat. No. CA5-H82F4) with a linear range of 0.5-16 ng/mL (Routinely tested).
Protocol
活性（Bioactivity）-BLI
Loaded Monoclonal Anti-Human MSLN Antibody, Human IgG1 on Protein A Biosensor, can bind Rat Mesothelin (298-600), His Tag (Cat. No. MSN-R52H4) with an affinity constant of 301 nM as determined in BLI assay (ForteBio Octet Red96e) (Routinely tested).
Protocol
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