关注公众号
收藏产品 产品详情
分子别名（Synonym）
MSLN,Mesothelin,MPF
表达区间及表达系统（Source）
FITC-Labeled Human Mesothelin (296-580), His Tag (MSN-HF2H3) is produced via conjugation of FITC to Human Mesothelin (296-580), His Tag with a new generation site-specific technology under Star Staining labeling platform. Human Mesothelin (296-580), His Tag is expressed from human 293 cells (HEK293). It contains AA Glu 296 - Gly 580 (Accession # AAH09272.1).
Predicted N-terminus: Glu 296
Request for sequence蛋白结构（Molecular Characterization）
This protein carries a polyhistidine tag at the C-terminus.
The protein has a calculated MW of 48.5 kDa. The protein migrates as 50-65 kDa when calibrated against Star Ribbon Pre-stained Protein Marker under reducing (R) condition (SDS-PAGE) due to glycosylation.
偶联（Conjugate）
FITC
Excitation source: 488 nm spectral line, argon-ion laser
Excitation Wavelength: 488 nm
Emission Wavelength: 535 nm
纯度（Purity）
>90% as determined by SDS-PAGE.
制剂（Formulation）
Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.
Contact us for customized product form or formulation.
重构方法（Reconstitution）
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
存储（Storage）
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please protect from light and avoid repeated freeze-thaw cycles.
This product is stable after storage at:
- -20°C to -70°C for 12 months in lyophilized state;
- -70°C for 3 months under sterile conditions after reconstitution.
质量管理控制体系（QMS）
数据展示
电泳（SDS-PAGE）
FITC-Labeled Human Mesothelin (296-580), His Tag on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 90% (With Star Ribbon Pre-stained Protein Marker).
CAR阳性表达率检测（Evaluation of CAR expression）
FACS Analysis of Anti-Mesothelin CAR Expression
2e5 of 293 CAR cells transfected with anti-MSLN-scFv were stained with 100 μL of 3 μg/mL of FITC-Labeled Human Mesothelin (296-580), His Tag (Cat. No. MSN-HF2H3) and negative control protein respectively (Fig. C and B), and non- transfected 293 cells were used as a control (Fig. A), FITC signal was used to evaluate the binding activity (QC tested).
FACS Analysis of Non-specific binding to PBMCs
5e5 of PBMCs were stained with FITC-Labeled Human Mesothelin (296-580), His Tag (Cat. No. MSN-HF2H3) and anti-CD3 antibody, washed and then analyzed with FACS. PE signal was used to evaluate the expression of CD3+ T cells in PBMCs, and FITC signal was used to evaluate the non-specific binding activity to PBMCs (QC tested).
用户评价 发表评论
药物研发进展 
联系我们 
项目合作