Human Matriptase / ST14 Catalytic Domain Protein, Tag Free (active enzyme)

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MAE-H5143-100ug
¥3675.00
MAE-H5143-1mg
¥24255.00
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产品详情

  • 分子别名(Synonym)

    Matriptase

  • 表达区间及表达系统(Source)

    Human Matriptase / ST14 Catalytic Domain Protein, Tag Free (MAE-H5143) is expressed from E. coli cells. It contains AA Gly 596 - Val 855 (Accession # NP_068813).

    Predicted N-terminus: Met

    Request for sequence
  • 蛋白结构(Molecular Characterization)

    Matriptase Structure

    Other Tags and Version Biotin & Other Labeled Version

    This protein carries no "tag".

    The protein has a calculated MW of 30.6 kDa. The protein migrates as 27-28 kDa when calibrated against Star Ribbon Pre-stained Protein Marker under reducing (R) condition (SDS-PAGE).

  • 纯度(Purity)

    >90% as determined by SDS-PAGE.

  • 制剂(Formulation)

    Supplied as 0.2 μm filtered solution in 50 mM Tris, 150 mM NaCl, 10 % Trehalose, pH7.5 with glycerol as protectant.

    Contact us for customized product form or formulation.

  • 运输(Shipping)

    This product is supplied and shipped with dry ice, please inquire the shipping cost.

  • 存储(Storage)

    Please avoid repeated freeze-thaw cycles.

    This product is stable after storage at:

    1. The product MUST be stored at -70°C or lower upon receipt;
    2. -70°C for 3 months under sterile conditions.
  • 质量管理控制体系(QMS)

    1. 质量管理体系(ISO, GMP)
    2. 质量优势
    3. 质控流程

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数据展示

  • 电泳(SDS-PAGE)

    Matriptase SDS-PAGE

    Human Matriptase / ST14 Catalytic Domain Protein, Tag Free on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 90% (With Star Ribbon Pre-stained Protein Marker).

  • 活性(Bioactivity)

    Measured by its ability to cleave the fluorogenic peptide substrate Boc-QAR-AMC . The specific activity is >15,000 pmol/min/μg (QC tested).

    Protocol

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背景介绍

Degrades extracellular matrix. Proposed to play a role in breast cancer invasion and metastasis. Exhibits trypsin-like activity as defined by cleavage of synthetic substrates with Arg or Lys as the P1 site. Involved in the terminal differentiation of keratinocytes through prostasin (PRSS8) activation and filaggrin (FLG) processing; Cleaves various synthetic substrates with Arg or Lys at the P1 position and prefers small side-chain amino acids, such as Ala and Gly, at the P2 position.

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