FITC-Labeled Human LILRB4 / CD85k / ILT3 Protein, Fc Tag

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LI4-HF254-25ug
¥3350.00
LI4-HF254-200ug
¥20790.00
合计0件 产品金额¥ 0

产品详情

  • 分子别名(Synonym)

    LILRB4, ILT3, LIR5, CD85K, HM18

  • 表达区间及表达系统(Source)

    FITC-Labeled Human LILRB4 Protein, Fc Tag (LI4-HF254) is expressed from human 293 cells (HEK293). It contains AA Gln 22 - Glu 259 (Accession # AAH26309.1). It is the FITC labeled form of Human LILRB4, Fc Tag.

    Predicted N-terminus: Gln 22

    Request for sequence
  • 蛋白结构(Molecular Characterization)

    LILRB4 Structure

    This protein carries a human IgG1 Fc tag at the C-terminus.

    The protein has a calculated MW of 52.9 kDa. The protein migrates as 66 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

  • 偶联(Conjugate)

    FITC

    Excitation source: 488 nm spectral line, argon-ion laser

    Excitation Wavelength: 488 nm

    Emission Wavelength: 535 nm

  • 标记(Labeling)

    The primary amines in the side chains of lysine residues and the N-terminus of the protein are conjugated with FITC using standard chemical labeling method. The residual FITC is removed by molecular sieve treatment during purification process.

  • 蛋白标记度(Protein Ratio)

    The FITC to protein molar ratio is 1.5-3.5.

  • 纯度(Purity)

    >90% as determined by SDS-PAGE.

  • 制剂(Formulation)

    Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.

    Contact us for customized product form or formulation.

  • 重构方法(Reconstitution)

    Please see Certificate of Analysis for specific instructions.

    For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

  • 存储(Storage)

    For long term storage, the product should be stored at lyophilized state at -20°C or lower.

    Please protect from light and avoid repeated freeze-thaw cycles.

    This product is stable after storage at:

    1. -20°C to -70°C for 12 months in lyophilized state;
    2. -70°C for 3 months under sterile conditions after reconstitution.
  • 质量管理控制体系(QMS)

    1. 质量管理体系(ISO, GMP)
    2. 质量优势
    3. 质控流程

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数据展示

  • 电泳(SDS-PAGE)

    LILRB4 SDS-PAGE

    FITC-Labeled Human LILRB4 Protein, Fc Tag on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 90%.

  • 活性(Bioactivity)-ELISA

     LILRB4 ELISA

    Immobilized Anti-LILRB4 Antibody, Human IgG1 at 1 μg/mL (100 μL/well) can bind FITC-Labeled Human LILRB4 Protein, Fc Tag (Cat. No. LI4-HF254) with a linear range of 0.01-0.313 μg/mL (QC tested).

    Protocol

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背景介绍

Leukocyte immunoglobulin-like receptor subfamily B member 4 (LILRB4) is also known as CD85 antigen-like family member K (CD85K), Immunoglobulin-like transcript 3 (ILT-3), Leukocyte immunoglobulin-like receptor 5 (LIR-5), Monocyte inhibitory receptor HM18, which belongs to the leukocyte immunoglobulin-like receptor (LIR) family. LILRB4 / CD85K contains 2 Ig-like C2-type (immunoglobulin-like) domains. CD85K is detected in monocytes, macrophages, dendritic cells, lung, natural killer cells and B-cells. LILRB4 / CD85K is receptor for class I MHC antigens. CD85K recognizes a broad spectrum of HLA-A, HLA-B, HLA-C and HLA-G alleles, involved in the down-regulation of the immune response and the development of tolerance. LILRB4 interferes with TNFRSF5-signaling and NF-kappa-B up-regulation and inhibits receptor-mediated phosphorylation of cellular proteins and mobilization of intracellular calcium ions.

前沿进展

 
药物研发进展
  • 英文全称:

    Leukocyte immunoglobulin-like receptor subfamily B member 4

  • 中文全称:

    免疫抑制受体LILRB4

  • 种类:

  • 上市药物数量:

    0 详情

  • 临床药物数量:

    6 详情

  • 最高研发阶段:

    临床二期

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