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分子别名（Synonym）
LIFR, CD118, FLJ98106, FLJ99923, SJS2, STWS, SWS
表达区间及表达系统（Source）
Human LIF R, Fc Tag (LIR-H4252) is expressed from human 293 cells (HEK293). It contains AA Gln 45 - Ser 833 (Accession # P42702-1).
Predicted N-terminus: Gln 45
Request for sequence蛋白结构（Molecular Characterization）
This protein carries a human IgG1 Fc tag at the C-terminus.
The protein has a calculated MW of 115.5 kDa. The protein migrates as 140-190 kDa when calibrated against Star Ribbon Pre-stained Protein Marker under reducing (R) condition (SDS-PAGE) due to glycosylation.
内毒素（Endotoxin）
Less than 1.0 EU per μg by the LAL method / rFC method.
纯度（Purity）
>95% as determined by SDS-PAGE.
制剂（Formulation）
Lyophilized from 0.22 μm filtered solution in Tris with Glycine, Arginine and NaCl, pH7.5 with trehalose as protectant.
Contact us for customized product form or formulation.
重构方法（Reconstitution）
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
存储（Storage）
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
- -20°C to -70°C for 12 months in lyophilized state;
- -70°C for 3 months under sterile conditions after reconstitution.
质量管理控制体系（QMS）
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数据展示
电泳（SDS-PAGE）
Human LIF R, Fc Tag on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 95% (With Star Ribbon Pre-stained Protein Marker).
活性（Bioactivity）-ELISA
Immobilized ActiveMax® Human LIF, Tag Free (Cat. No. LIF-H521b) at 0.2 μg/mL (100 μL/well) can bind Human LIF R, Fc Tag (Cat. No. LIR-H4252) with a linear range of 4-125 ng/mL (QC tested).
Protocol
活性（Bioactivity）-SPR
Human LIF R Protein, Fc Tag (Cat. No. LIR-H4252) captured on Protein A Chip can bind Human Oncostatin M (OSM) (26-221) Protein, premium grade (Cat. No. OSM-H5214) with an affinity constant of 595 nM as determined in a SPR assay (Biacore 8K) (Routinely tested).
Protocol
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