Mouse Legumain Protein, His Tag (active enzyme, HPLC verified)

用户评价
货号-规格
价格
Qty.
LEN-M52H3-100ug
¥3500.00
LEN-M52H3-1mg
¥25000.00
合计0件 产品金额¥ 0

产品详情

  • 分子别名(Synonym)

    AEP, LGMN1, PRSC1

  • 表达区间及表达系统(Source)

    Mouse Legumain Protein, His Tag (LEN-M52H3) is expressed from human 293 cells (HEK293). It contains AA Val 18 - Tyr 435 (Accession # O89017-1).

    Predicted N-terminus: Val 18

    Request for sequence
  • 蛋白结构(Molecular Characterization)

    Legumain Structure

    This protein carries a polyhistidine tag at the C-terminus.

    The protein has a calculated MW of 49.4 kDa. The protein migrates as 43-48 kDa and 50-60 kDa when calibrated against Star Ribbon Pre-stained Protein Marker under reducing (R) condition (SDS-PAGE) due to glycosylation.

  • 内毒素(Endotoxin)

    Less than 1.0 EU per μg by the LAL method / rFC method.

  • 纯度(Purity)

    >95% as determined by SDS-PAGE.

    >90% as determined by SEC-HPLC.

  • 制剂(Formulation)

    Supplied as 0.2 μm filtered solution in 20 mM Tris, 20 mM NaCl, pH7.5 with glycerol as protectant.

    Contact us for customized product form or formulation.

  • 运输(Shipping)

    This product is supplied and shipped with dry ice, please inquire the shipping cost.

  • 存储(Storage)

    Please avoid repeated freeze-thaw cycles.

    This product is stable after storage at:

    1. The product MUST be stored at -70°C or lower upon receipt;
    2. -70°C for 3 months under sterile conditions.
  • 质量管理控制体系(QMS)

    1. 质量管理体系(ISO, GMP)
    2. 质量优势
    3. 质控流程

推荐产品

数据展示

  • 电泳(SDS-PAGE)

    Legumain SDS-PAGE

    Mouse Legumain Protein, His Tag on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 95% (With Star Ribbon Pre-stained Protein Marker).

  • SEC-HPLC

    Legumain SEC-HPLC

    The purity of Mouse Legumain Protein, His Tag (Cat. No. LEN-M52H3) was greater than 90% as determined by SEC-HPLC.

  • 活性(Bioactivity)

    Measured by its ability to cleave the fluorogenic peptide substrate, N-carbobenzyloxy-Ala-Ala-Asn-7-amido-4-methylcoumarin (Z-AAN-AMC). The specific activity is >350 pmol/min/μg (QC tested).

    Protocol

用户评价
发表评论

背景介绍

This gene encodes a member of the cysteine peptidase family C13 that plays an important role in the endosome/lysosomal degradation system. The encoded inactive preproprotein undergoes autocatalytic removal of the C-terminal inhibitory propeptide to generate the active endopeptidase that cleaves protein substrates on the C-terminal side of asparagine residues. Mice lacking the encoded protein exhibit defects in the lysosomal processing of proteins resulting in their accumulation in the lysosomes, and develop symptoms resembling hemophagocytic lymphohistiocytosis.

前沿进展

 
联系我们
项目合作
查看更多项目
实验案例分析
查看更多案例分析
  • 产品基础信息
  • 产品详情
  • 推荐产品
  • 数据展示
  • 用户评价
  • 背景介绍