Biotinylated Human LC3B Protein, Avitag™,His Tag (MALS verified)

分子别名（Synonym）

LC3B

表达区间及表达系统（Source）

Biotinylated Human LC3B Protein, Avitag,His Tag (LCB-H82Q3) is expressed from E. coli cells. It contains AA Met 1 - Val 125 (Accession # Q9GZQ8).

Predicted N-terminus: Met

蛋白结构（Molecular Characterization）

Other Tags and Version Biotin & Other Labeled Version

This protein carries an Avi tag (Avitag™) at the N-terminus, followed by a polyhistidine tag.

The protein has a calculated MW of 18.4 kDa. The protein migrates as 19-20 kDa when calibrated against Star Ribbon Pre-stained Protein Marker under reducing (R) condition (SDS-PAGE).

标记（Labeling）

Biotinylation of this product is performed using Avitag™ technology. Briefly, the single lysine residue in the Avitag is enzymatically labeled with biotin.

蛋白标记度（Protein Ratio）

Passed as determined by the HABA assay / binding ELISA.

纯度（Purity）

>90% as determined by SDS-PAGE.

>95% as determined by SEC-MALS.

制剂（Formulation）

Lyophilized from 0.22 μm filtered solution in 20 mM Citric acid, 200 mM NaCl, pH3.5 with trehalose as protectant.

Contact us for customized product form or formulation.

重构方法（Reconstitution）

Please see Certificate of Analysis for specific instructions.

For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

存储（Storage）

For long term storage, the product should be stored at lyophilized state at -20°C or lower.

Please avoid repeated freeze-thaw cycles.

This product is stable after storage at:

1. -20°C to -70°C for 12 months in lyophilized state;
2. -70°C for 3 months under sterile conditions after reconstitution.

质量管理控制体系（QMS）

1. 质量管理体系（ISO, GMP）
2. 质量优势
3. 质控流程