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分子别名（Synonym）
LAG3, CD223, FDC
表达区间及表达系统（Source）
Human LAG-3 / CD223 Protein, His Tag (LA3-H52H3) is expressed from human 293 cells (HEK293). It contains AA Leu 23 - Leu 450 (Accession # P18627-1).
Predicted N-terminus: Leu 23
Request for sequence蛋白结构（Molecular Characterization）
This protein carries a polyhistidine tag at the C-terminus.
The protein has a calculated MW of 48.1 kDa. The protein migrates as 58-65 kDa when calibrated against Star Ribbon Pre-stained Protein Marker under reducing (R) condition (SDS-PAGE) due to glycosylation.
内毒素（Endotoxin）
Less than 1.0 EU per μg by the LAL method / rFC method.
纯度（Purity）
>90% as determined by SDS-PAGE.
>95% as determined by SEC-MALS.
制剂（Formulation）
Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.
Contact us for customized product form or formulation.
重构方法（Reconstitution）
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
存储（Storage）
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
- -20°C to -70°C for 12 months in lyophilized state;
- -70°C for 3 months under sterile conditions after reconstitution.
质量管理控制体系（QMS）
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数据展示
电泳（SDS-PAGE）
Human LAG-3 / CD223 Protein, His Tag on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 90% (With Star Ribbon Pre-stained Protein Marker).
SEC-MALS
The purity of Human LAG-3 / CD223 Protein, His Tag (Cat. No. LA3-H52H3) is more than 95% and the molecular weight of this protein is around 60-85 kDa verified by SEC-MALS.
Report
活性（Bioactivity）-ELISA
Immobilized Human LAG-3 / CD223 Protein, His Tag (Cat. No. LA3-H52H3) at 2 μg/mL (100 μL/well) can bind Monoclonal Anti-Human LAG3 Antibody, Human IgG1 with a linear range of 0.1-4 ng/mL (QC tested).
Protocol
Immobilized Human LAG-3 / CD223 Protein, His Tag (Cat. No. LA3-H52H3) at 2 μg/mL (100 μL/well) can bind Monoclonal Anti-Human LAG3 Antibody, Human IgG4 (S108P) with a linear range of 0.2-4 ng/mL (Routinely tested).
Protocol
Immobilized Human LAG-3 / CD223 Protein, His Tag (Cat. No. LA3-H52H3) at 1 μg/mL (100 μL/well) can bind Anti-LAG3 Antibody, Human IgG1 with a linear range of 0.06-2 ng/mL (Routinely tested).
Protocol
Immobilized Human LAG-3 / CD223 Protein, His Tag (Cat. No. LA3-H52H3) at 5 μg/mL, add Anti-PD1 crossFab x LAG3, Human IgG1 and then add Biotinylated Human PD-1, Fc,Avitag (Cat. No. PD1-H82F1) at 0.2 μg/mL. Detection was performed using HRP-conjugated Streptavidin (Acro, Cat. No. STN-NH913) (Routinely tested).
Protocol
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