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分子别名（Synonym）
betaKlotho, beta-klotho, BKL, KLB, klotho beta like, klotho beta-like protein
表达区间及表达系统（Source）
Cynomolgus / Rhesus macaque Klotho beta Protein, His Tag (KLB-R52H8) is expressed from human 293 cells (HEK293). It contains AA Phe 53 - Leu 997 (Accession # G7MSR8-1).
Predicted N-terminus: Phe 53
Request for sequence蛋白结构（Molecular Characterization）
This protein carries a polyhistidine tag at the C-terminus.
The protein has a calculated MW of 110.3 kDa. The protein migrates as 115-130 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.
内毒素（Endotoxin）
Less than 1.0 EU per μg by the LAL method / rFC method.
纯度（Purity）
>95% as determined by SDS-PAGE.
制剂（Formulation）
Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.
Contact us for customized product form or formulation.
重构方法（Reconstitution）
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
存储（Storage）
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
- -20°C to -70°C for 12 months in lyophilized state;
- -70°C for 3 months under sterile conditions after reconstitution.
质量管理控制体系（QMS）
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数据展示
电泳（SDS-PAGE）
Cynomolgus / Rhesus macaque Klotho beta Protein, His Tag on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 95%.
活性（Bioactivity）-BLI
Loaded Human FGF R4, Fc Tag (Cat. No. FG4-H5253) on Protein A Biosensor, can bind Cynomolgus / Rhesus macaque Klotho beta Protein, His Tag (Cat. No. KLB-R52H8) with an affinity constant of 0.9 μM as determined in BLI assay (ForteBio Octet Red96e) (QC tested).
Protocol
Loaded Human FGF19, Fc Tag (Cat. No. FG9-H5253) on Protein A Biosensor, can bind Cynomolgus / Rhesus macaque Klotho beta Protein, His Tag (Cat. No. KLB-R52H8) with an affinity constant of 78.5 nM as determined in BLI assay (ForteBio Octet Red96e) (QC tested).
Protocol
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