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分子别名（Synonym）
KLK2A2, hGK-1, hK2
表达区间及表达系统（Source）
Biotinylated Human Kallikrein 2 Protein, His,Avitag (KA2-H82E3) is expressed from human 293 cells (HEK293). It contains AA Ile 25 - Pro 261 (Accession # P20151-1).
Predicted N-terminus: Ile 25
Request for sequence蛋白结构（Molecular Characterization）
This protein carries a polyhistidine tag at the C-terminus, followed by an Avi tag (Avitag™).
The protein has a calculated MW of 29.9 kDa. The protein migrates as 12 kDa, 19 kDa and 30-33 kDa when calibrated against Star Ribbon Pre-stained Protein Marker under reducing (R) condition (SDS-PAGE) due to glycosylation.
标记（Labeling）
Biotinylation of this product is performed using Avitag™ technology. Briefly, the single lysine residue in the Avitag is enzymatically labeled with biotin.
蛋白标记度（Protein Ratio）
Passed as determined by the HABA assay / binding ELISA.
内毒素（Endotoxin）
Less than 1.0 EU per μg by the LAL method / rFC method.
纯度（Purity）
>85% as determined by SDS-PAGE.
制剂（Formulation）
Supplied as 0.2 μm filtered solution in 50 mM Tris, 150 mM NaCl, pH7.5 with glycerol as protectant.
Contact us for customized product form or formulation.
运输（Shipping）
This product is supplied and shipped with dry ice, please inquire the shipping cost.
存储（Storage）
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
- The product MUST be stored at -70°C or lower upon receipt;
- -70°C for 3 months under sterile conditions.
质量管理控制体系（QMS）
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数据展示
电泳（SDS-PAGE）
Biotinylated Human Kallikrein 2 Protein, His,Avitag on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 85% (With Star Ribbon Pre-stained Protein Marker).
活性（Bioactivity）-ELISA
Immobilized Biotinylated Human Kallikrein 2 Protein, His,Avitag (Cat. No. KA2-H82E3) at 1 μg/mL (100 μL/well) on streptavidin (Cat. No. STN-N5116) precoated (0.5 μg/well) plate can bind Anti-KLK2 Antibody (Pasritamig Biosimilar) with a linear range of 0.06-2 ng/mL (Routinely tested).
Protocol
活性（Bioactivity）-SPR
Pasritamig captured on Protein A Chip can bind Biotinylated Human Kallikrein 2 Protein, His,Avitag (Cat. No. KA2-H82E3) with an affinity constant of 7.65 nM as determined in a SPR assay (Biacore 8K) (Routinely tested).
Protocol
活性（Bioactivity）
Measured by its ability to cleave a fluorogenic peptide substrate Pro-Phe-Arg-7-amido-4-methylcoumarin (PFR-AMC). The specific activity is >250 pmol/min/μg (QC tested).
Protocol
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