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分子别名（Synonym）
KLK2A2, hGK-1, hK2
表达区间及表达系统（Source）
Human Kallikrein 2 Protein, Tag Free (KA2-H5214) is expressed from human 293 cells (HEK293). It contains AA Ile 25 - Pro 261 (Accession # P20151-1).
Predicted N-terminus: Ile 25
Request for sequence蛋白结构（Molecular Characterization）
This protein carries no "tag".
The protein has a calculated MW of 26.2 kDa. The protein migrates as 30-31 kDa when calibrated against Star Ribbon Pre-stained Protein Marker under reducing (R) condition (SDS-PAGE) due to glycosylation.
内毒素（Endotoxin）
Less than 0.2 EU per μg by the LAL method / rFC method.
纯度（Purity）
>95% as determined by SDS-PAGE.
制剂（Formulation）
Supplied as 0.2 μm filtered solution in 50 mM Tris, 150 mM NaCl, pH7.5 with glycerol as protectant.
Contact us for customized product form or formulation.
运输（Shipping）
This product is supplied and shipped with dry ice, please inquire the shipping cost.
存储（Storage）
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
- The product MUST be stored at -70°C or lower upon receipt;
- -70°C for 3 months under sterile conditions.
质量管理控制体系（QMS）
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数据展示
电泳（SDS-PAGE）
Human Kallikrein 2 Protein, Tag Free on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 95% (With Star Ribbon Pre-stained Protein Marker).
活性（Bioactivity）-ELISA
Immobilized Human Kallikrein 2 Protein, Tag Free (Cat. No. KA2-H5214) at 1 μg/mL (100 μL/well) can bind Human KLK2 Antibody with a linear range of 0.1-8 ng/mL (Routinely tested).
Protocol
Immobilized Human Kallikrein 2 Protein, Tag Free (Cat. No. KA2-H5214) at 1 μg/mL (100 μL/well) can bind Anti-KLK2 Antibody (Pasritamig Biosimilar) with a linear range of 0.06-2 ng/mL (Routinely tested).
Protocol
活性（Bioactivity）-SPR
Pasritamig captured on Protein A Chip can bind Human Kallikrein 2 Protein, Tag Free (Cat. No. KA2-H5214) with an affinity constant of 4.87 nM as determined in a SPR assay (Biacore 8K) (Routinely tested).
Protocol
活性（Bioactivity）
Measured by its ability to cleave a flourogenic peptide substrate Pro-Phe-Arg-7-amido-4-methylcoumarin (PFR-AMC). The specific activity is >450 pmol/min/µg (QC tested).
Protocol
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