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分子别名（Synonym）
Integrin alpha V beta 8, ITGAV&ITGB8
表达区间及表达系统（Source）
Rat Integrin alpha V beta 8 (ITGAV&ITGB8) Heterodimer, His Tag&Tag Free (IT8-R52E1) is expressed from human 293 cells (HEK293). It contains AA Phe 31 - Pro 988 & Gly 22 - Ser 679 (Accession # NP_001385621.1 & A0A0G2JVU1).
Predicted N-terminus: Phe 31 | Gly 22
Request for sequence蛋白结构（Molecular Characterization）
Rat ITGAV&ITGB8 Heterodimer Protein, His Tag&Tag Free, produced by co-expression of ITGAV and ITGB8, has a calculated MW of 113 kDa (ITGAV) & 77 kDa (ITGB8). Subunit ITGAV is fused with an acidic tail at the C-terminus and followed by a polyhistidine tag and subunit ITGB8 contains no tag but a basic tail at the C-terminus. The protein migrates as 125-145 kDa (ITGAV) and 75-85 kDa (ITGB8) under non-reducing (NR) condition (SDS-PAGE) due to glycosylation.
内毒素（Endotoxin）
Less than 1.0 EU per μg by the LAL method / rFC method.
纯度（Purity）
>95% as determined by SDS-PAGE.
>90% as determined by SEC-MALS.
制剂（Formulation）
Lyophilized from 0.22 μm filtered solution in 50 mM Tris, 150 mM NaCl, pH7.5 with trehalose as protectant.
Contact us for customized product form or formulation.
重构方法（Reconstitution）
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
存储（Storage）
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
- -20°C to -70°C for 12 months in lyophilized state;
- -70°C for 3 months under sterile conditions after reconstitution.
质量管理控制体系（QMS）
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数据展示
电泳（SDS-PAGE）
Rat Integrin alpha V beta 8 (ITGAV&ITGB8) Heterodimer, His Tag&Tag Free on SDS-PAGE under non-reducing (NR) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 95%.
SEC-MALS
The purity of Rat Integrin alpha V beta 8 (ITGAV&ITGB8) Heterodimer, His Tag&Tag Free (Cat. No. IT8-R52E1) is more than 90% and the molecular weight of this protein is around 200-230 kDa verified by SEC-MALS.
Report
活性（Bioactivity）-ELISA
Immobilized Rat Integrin alpha V beta 8 (ITGAV&ITGB8) Heterodimer, His Tag&Tag Free (Cat. No. IT8-R52E1) at 5 μg/mL (100 μL/well) can bind Biotinylated Human Latent TGF-Beta 1, His,Avitag (Cat. No. TG1-H82Qb) with a linear range of 0.2-13 ng/mL (QC tested).
Protocol
Immobilized Rat Integrin alpha V beta 8 (ITGAV&ITGB8) Heterodimer, His Tag&Tag Free (Cat. No. IT8-R52E1) at 5 μg/mL (100 μL/well) can bind Biotinylated Human Vitronectin with a linear range of 0.1-3 ng/mL (Routinely tested).
Protocol
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